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TITLE
DECLARATION
ACKNOWLEDGEMENT
ABSTRACT
PREFACE
CONTENTS
LIST OF TABLES
LIST OF FIGURES
LIST OF PLATES
ABBREVIATIONS
1 INTRODUCTION
1.1. Nature of xylan and xylanases
Fig.1.1: A hypothetical plant xylan structure showing different substitient groups with sites of attack by microbial xylanases
1.2. REVIEW OF LITERATURE
Table 1. 2: Sources of microbial xylanases
Table 1.3: Production of xylanases in solid state-fermentation by microorganisms
1.3. OBJECTIVES OF THE PRESENT STUDY
2 MATERIALS AND METHODS
2.1. Isolation of fungi
2.2. Screening for xylanase production using Czapek’s Agar Medium
2.3. Identification of xylanolytic fungi
2.4. Xylanase production of selected strains by solid state fermentations
2.5. Preparation of inoculum
2.6. Inoculation and incubation
2.7. Enzyme recovery
2.8. Xylanase production of selected strains by submerged fermentation
2.9. Enzyme recovery
2.10. Estimation of Biomass
2.11. Enzyme assays
2.12. Microorganism
2.13. Effect of different physico-chemical parameters on the production of xylanase from Aspergillus sydowii SBS45
2.14. Purification of xylanase I and Xylanase II fom Aspergillus Sydowii SBS 45
2.15. Poly Acrylamide Gel Electrophoresis (PAGE)
2.16. Characterisation of purified xylanase I and xylanase II
2.17. Application of crude xylanase from Aspergillus sydowii SBS45 in the pulp and paper industry
3 RESULTS
3.1. Isolation and screening of xylan-degrading fungi
Plate 3.1: Growth of fungal strains
3.2. Xylanase production by solid state fermentation (SSF)
3.3. Xylanase production by submerged fermentation (SmF)
3.4. Characteristic features of Aspergillus sydowii SBS 45
Plate 3.2: Photograph of seven day old slide culture of Aspergillussydowii SBS 45
3.5. Effect of different physico-chemical parameters on theproduction of xylanase from Aspergillus sydowii SBS 45
Fig.3.6: Effect of sugars and non-sugars on the production of xylanase from Aspergillus sydowii SBS 45under SmF
Fig.3.9: Effect of various inorganic and organic nitrogen sources on the production of xylanase from Aspergillus sydowii SBS 45 under SmF
3.6. Production of xylanase in optimised condition
3.7. Purification of xylanase I and xylanase II from Aspergillus sydowii SBS 45
3.8. Characterisation studies of xylanase I and xylanase IIisolated from Aspergillus sydowii SBS 45
3.9. Total carbohydrate content of xylanase I and xylanase II from Aspergillus sydowii SBS 45
3.10. Application of crude xylanase form Aspergillus sydowii SBS45 in the pulp and paper industry
4 DISCUSSION
4.1. Isolation, screening and identification of xylan-degrading fungi
4.2. Xylanase production of selected strains by solid statefermentation (SSF) and submerged fermentation (SmF)
4.3. Effect of different physico-chemical parameters on the production of xylanase from Aspergillus sydowii SBS 45
4.4. Purification of xylanases from Aspergillus sydowii SBS45
4.5. Characterisation studies of purified xylanase I and Xylanase II
4.6. Application of crude xylanase from Aspergillus sydowii SBS 45 in the pulp and paper industry
5 SUMMARY
REFERENCES