Online Thesis Search Results

TITLE
CERTIFICATE
DECLARATION
DEDICATION
ACKNOWLEDGEMENT
CONTENTS
ABBREVIATIONS
1. INTRODUCTION AND REVIEW OF LITERATURE
INTRODUCTION
REVIEW OF LITERATURE
1.1. THE HALLMARKS OF CANCER
Fig.1.1.Different acquired capabilities of cancer
1.2. CELL CYCLE AND GROWTH OF CANCER CELLS
1.3. METASTASIS AND CANCER
1.4. MOLECULAR BASIS OF CANCER
1.5. GROWTH FACTORS AND CANCER
1.6. CARCINOGENESIS
1.7. REACTIVE OXYGEN SPECIES AND CANCER
Fig. 1.2. Different ways in which singlet oxygen can be generated
1.8. CANCER THERAPY
1.9. PLANT POLYPHENOLS AS A CLASS OF NATURAL THERAPEUTIC DRUGS.
1.10. PRODUCTION OF ANTI NEOPLASTIC SECONDARY METABOLITES THROUGH PLANT BIOTECHNOLOGY
Fig. 1.5. Simplified model for network of secondary metabolite biosynthesis; E = Enzyme, S = Product
Fig. 1.6. Compartmentlization of alkaloid biosynthesis in Catharanthus roseus; all steps are shown as occuring in a single cell.
2. MATERIALS AND METHODS
2.1. MATERIALS
2.2. METHODS
2.3. Cytotoxicity assays
2.4. Topoisomerase I and II inhibition assay
2.5. Propagation of tumour cell lines
2.6. Determination of tumour reducing activity of plant products
2.7. Determination of in vitro antioxidant activity
2.8. Estimation of protein
2.9. Estimation of tissue glutathione (GSH)
2.10. Estimation of serum glutamate -pyruvate transaminase (GPT) activity
2.11. Estimation of Alkaline phosphatase (ALP) activity
2.12. Histopathology
2.13. Statistical analysis
3. ISOLATION AND QUANTIFICATION OF CAMPTOTHECIN FROM OPHIORRHIZA RUGOSA VAR.DECUMBENS
3.1. INTRODUCTION
Plate No.1. Ophiorrhiza rugosa var. decumbens (Deb and Mondel)
3.2. MATERIALS AND METHODS
Fig 3.1. Schematic representation for the isolation of CPT from plant parts of O.rugosa var. decumbens
Fig.3.2.a. UV Visible absorption spectra of standard CPT Fig.3.2.b. UV Visible absorption spectra of isolated CPT
Fig.3.3.a. to Fig.3.3.c.
Fig.3.3.d. to Fig.3.3.i
Fig.3.3.j. to Fig.3.3.n.
Fig. 3.4.a. Mass spectrum of standard CPT
Fig. 3.4.b. Mass spectrum of isolated CPT
Fig. 3.5.a. NMR spectrum of standard CPT
Fig. 3.5.b. NMR spectrum of isolated CPT
Fig. 3.6.a. IR spectrum of standard CPT
Fig. 3.6.b. IR spectrum of isolated CPT
Fig. 3.7. A comaprison of cytotoxicity between isolated and standard camptothecin
Fig. 3.8. Schematic representation for the isolation of 10 - hydroxy CPT from plant parts of O.rugosa var. decumbens
Fig.3.9.a; b. Mass spectrum of 10-hydroxy CPT isolated from O.rugosa var.decumbens,
Fig.3.10.a. 1H NMR spectrum of anthraquinone isolated from O.rugosa var.decumbens,
3.3. RESULTS AND DISCUSSION
4. BIOTECHNOLOGICAL PRODUCTION OF CAMPTOTHECIN - A FEASIBLE APPROACH
4.1. INTRODUCTION
4.2. MATERIALS AND METHODS
Fig 4.1 a Camptothecin extraction from callus of O.rugosa var. decumbens
4.3. RESULTS AND DISCUSSION
Plate No.2. A. Emergence of multiple shoot from leaf explants
Plate No.3. A- In Vitro grown callus from O.rugosa var. decumbens
Plate N0.4. - Agrobacerium mediated hairy root induction;
5. GOSSYPIN-A BIOACTIVE SECONDARY METABOLITE FROM HIBISCUS FURCATUS
5.1. INTRODUCTION
5.2. MATERIALS AND METHODS
5.2.1. Plant Material
5.2.2. Isolation of gossypin (Fig. 5.1a)
5.2.3. Determination of Melting Point
5.2.4. HPLC analysis and quantification
5.2.4.1. Standardization of HPLC for the estimation of Gossypin
5.2.5. IR Spectra
5.2.6. Electron Spray Mass spectrometry (ESMS)
5.2.7. 1H-nuclear magnetic resonance
5.2.8. Tissue culture studies of Hibiscusfurcahts
5.2.8.1. Culture environment
5.2.8.2. Culture initiation and callus culture establishment
5.2.8.3. Callus growth measurement
5.3. RESULTS AND DISCUSSION
Plate No. 5. A & B- Habitat view of Hibiscusfurcatus
1.TOXICITY STUDIES OF GOSSYPIN
6. PHARMACOLOGICAL ACTIVITY OF GOSSYPIN
6.1.1. INTRODUCTION
6.1.2. MATERIALS AND METHODS
6.1.2.1. Animals
6.1.2.2. Acute toxicity study
6.1.3. RESULTS AND DISCUSSION
Plate No.6.
2.ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITY OF GOSSYPIN
6.2.1. iNTRODUCTION
6.2.2. MATERIALS AND METHODS
6.2.2.1. Animals
6.2.2.2. Inhibition of superoxide radical scavenging activity
6.2.2.3. Hydroxyl radical scavenging activity
6.2.2.4. Inhibition of lipid peroxide formation
6.2.2.5. Nitric oxide radical scavenging activity
6.2.3. RESULTS AND DISCUSSION
Plate No.7. - Scctions ui liver in CCla induced acute hepatotoxicity study
3.ANTI TUMOUR AND ANTI CARCINOGENICACTIVITY OF GOSSYPIN
6.3.1. INTRODUCTION
6.3.2. MATERIALS AND METHODS
6.3.3. RESULTS AND DISCUSSION
Plate No.8.
Plate No.9.
ANTI INFLAMMATORY AND GASTRIC CYTOPROTECTIVE ACTIVITY
6.4.1. INTRODUCTION
6.4.2. MATERIALS AND METHODS
6.4.2.1. Ethanol induced gastric lesions
6.4.2.2. Aspirin induced gastric ulcer
6.4.2.3. Anti-inflammatory study
6.4.2.4. Carragenan induced paw oedema
6.4.2.5. Formalin induced paw oedema
6.4.2.6. Dextran induced paw oedema
6.4.2.7. Croton oil induced skin oedema
6.4.3. RESULTS AND DISCUSSION
Plate No. 10.
Plate No.11.
7. SUMMARY AND CONCLUSION
BIBILIOGRAPHY
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