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  • TITLE
  • DECLARATION
  • CERTIFICATE
  • ACKNOWLEDGEMENT
  • CONTENTS
  • 1. Introduction and Review of Literature
  • 1.1. Definition of lectin
  • 1.2. Historical background
  • Plate-1 Rosary Pea
  • Plate-2
  • 1.3. Functions of lectins
  • 1.4. Detection of lectins
  • 1.5. Distribution of plant lectins
  • 1.6. Distribution of animal lectins
  • 1.7. Carbohydrate specificity of lectins
  • 1.8. Isolation of lectins
  • 1.9. Physiochemical properties of lectins
  • 1.10. Lectins and immune system
  • 1.11. Lectin induced cytotoxicity
  • 1.12. Leetin histochemistry
  • 1.13. Ribosome inactivating proteins (RIPS)
  • 1.14. Structure of RIP II
  • Plate-3
  • 1.15. Molecular mechanisms of toxicity of RIP II
  • Plate-4 The Mechanism of Toxic Action of Abrin
  • 1.16. Abrus precatorius Linn
  • 1.17. Toxicity of abrin
  • 1.18. Mechanism of action of abrin
  • 1.19. Use of abrin as an adjuvant in cancer chemotherapy
  • 1.20. Use of abrin as immunotoxins
  • 1.21. Use of abrin as mitogen
  • 1.22. Abrin and apoptosis
  • 1.23. Aims and Objectives
  • 2. Materials and Methods
  • 2.1. Chemicals
  • 2.2. Diagnostic and reagent kits
  • 2.3. Reagents and stains
  • 2.4. Animals and maintenance
  • 2.5. Blood collection
  • 2.6. Plant materials
  • 2.7. Cell lines
  • 2.8. Instruments
  • 2.9. Haematological parameters
  • 2.10. Biochemical parameters
  • 2.11. Immunological parameters
  • 2.12. SDS-PAGE gel electrophoresis
  • 2.13. Isolation of DNA
  • 2.14. Agarose gel electrophoresir, for DNA / PCR products separation
  • 2.15. Statistical analysis
  • 3. Isolation and characterisation of abrin from Abrus precatorius, Linn.
  • 3.1. Introduction
  • 3.2. Materials and methods
  • 3.3. Results
  • Table 3.1 Protein recovery profile during abrin isolation and purification
  • 3.4. Discussion
  • Table 3.2 Haemagglutination assay on abrin isolation and purification
  • Plate-5 SDS-PAGE gel electrophoretic separation of Abrus toxins agglutinins
  • 4. Cytotoxicity, anti tumour activity and toxicity profile of abrin.
  • 4.1. Introduction
  • 4.2. Materials and methods
  • 4.3. Results
  • Fig 4.1. Effect of abrin administration on total leucocyte count of mice
  • Fig 4.2. Effect of abrin administration on serum bilirubin level of mice
  • Fig 4.3. Effect of abrin administration on serum alanine amino transferase activity of mice
  • Fig 4.4 Antiturnour effect of abrin on DLA induced solid tumour when administered simultaneously
  • Fig 4.5. Antitumour effect of abrin on EAC induced solid tumour when administered simultaneously
  • Fig.4.6.Antitumour effect of abrin on DLA induced developed solid tumour mass
  • Fig 4.7. Antitumour effect of abrin on EAC induced developed solid tumour mass
  • 4.4. Discussion
  • 5. Immunomodulatory activity of abrin
  • 5.1. Introduction
  • 5.2. Materials and methods
  • 5.3. Results
  • Fig.5.1Effect of abrin on plaque forming cells
  • Fig.5.2. Effect of abrin on NK cell activity
  • Fig 5.3. Effect of abrin an antibody dependent cellular cytotoxicity
  • Fig 5.4. Effect of abrin on antibody dependent complement mediated cytotoxicity
  • 5.4. Discussion
  • 6. Chemoprotective and radioprotective properties of abrin
  • 6.1. Introduction
  • 6.2. Materials and methods
  • 6.3. Results
  • Fig.6.1. Protective effect of abrin on CTX induced changes in bone marrow cellularity
  • Fig.6.2 Protective effect of abrin on CTX induced changes in haemoglobin content
  • Fig.6.3 Protective effect of abrin CTX induced changes in total leucocyte count
  • Fig.6.4. Protective effect of abrin on CTX induced changes in serum ALP activity
  • Fig.6.5. Protective effect of abrin on CTX induced changes in serum AST activity
  • Fig.6.6. Protective effect of abrin on CTX induced changes in liver ALP activity
  • Fig.6.7. Protective effect of abrin on gamma radiation induced changes in bone marrow cellularity
  • Fig. 6.8. Protective effect of abrin on gamma radiation induced changes haemoglobin content
  • Fig.6.9. Protective effect of abrin on gamma radiation induced changes in total leucocyte count
  • 6.4. Discussion
  • Fig.6.10. Protective effect of abrin on gamma radiation induced changes in serum ALP activity
  • Fig.6.11. Protective effect of abrin on gamma radiation induced changes in serum AST activity
  • Fig.6.12. Protective effect of abrin on gamma radiation induced changes in liver ALP activity
  • 7. Induction of apoptosis by abrin
  • 7.1. Introduction
  • 7.2. Materials and methods
  • 7.3. Results
  • Plate-6 Morphology of apoptotic and necrotic DLA cells
  • Plate-7 Morphology of metabolically active normal, apoptotic and necrotic DLA cells
  • 7.4. Discussion
  • Plate-8 DNA fragmentation of DLA cells
  • Plate-9 PCR- Gene expression
  • 8. Summary and Conclusion
  • BIBILIOGRAPHY
  • List of publications