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Thesis Details
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TITLE
DEDICATION
CERTIFICATE
DECLARATION
ACKNOWLEDGEMENT
ABBREVIATIONS
CONTENTS
1 INTRODUCTION
The Objectives of this Study
2 REVIEW OF LITERATURE
3 MATERIALS AND METHODS
3.1 Galactose Cataract
I. Control group
II. Experimental group
Ill. Vitamin E Supplemented group
3.2 Selenium Cataract
3.3 Preparation of lens extract
3.4 Collection and preservation of blood
Processing of blood samples (Beutler, 1975)
(a) Preparation of packed red cell
(b) Preparation of 1: 20 hemolysate
3.5 BIOCHEMICAL ANALYSIS
Hemoglobin (Beutler, 1975)
Protein (Lowry 1951)
Superoxide dismutase (EC 1.15.1.I) (Paoletti and Mocali, 1990)
Reduced glutathione (Beutler. 1986)
Glutathione reductase (ED 1.6.4.2) (Beutler, 1986)
Glutathione-S-transferase (EC 2.5.1.45) (Beutler, 1986)
Malondialdehyde: - (John A Buege and Stevan P. Aust 1978)
3.6 Glycoprotein
3.7 Sodium Dodesyl Sulphate polyacrylamide gel electrophoresis (SDS-PAGE) (Laemle 1970)
3.8 Statistical Analysis
4 RESULTS
Fig. 4.2.A Mean wet weight of lens (mg) in normal, cataractous and vitamin E supplemented rats in galactose induced cataract
Fig. 4.2.B Mean wet weight of lens (mg) in normal, cataractous and vitamin C supplemented rats in selenium induced cataract
Plate. 4.1.A. Control lens
Plate 4.1.B. Stage 1. Galactose cataract (1 0 days)
Plate. 4.1.C. Stage 2. Galactose cataract (20 days)
Plate. 4.1.D. Stage 3. Galactose cataract (30 days)
Plate.4.1.E. Selenium cataract after one month
4.3 Various stages of preventive effect of vitamin E on galactose cataract
Plate.4.3.A. Stage 1. (10 days)
Plate.4.3.B. Stage 2. (20 days)
Plate. 4.3.C. Stage 3. (30 days)
Plate. 4.3 D. Preventive effect of vitarninc on selenium cataract after one month
4.4 Oxidative Stress
4.5 Lipid Peroxidation
4.6 Glycoprotein
Plate 4.7.A Protein Profile of Galactose Induced Cataract affected Lens from 10 days old rat
4.7 Electrophoresis
Plate 4.7.B Protein Profile of Galactose Induced Cataract affected Lens from 20 days old rat
Plate 4.7.C Protein Profile of Galactose Induced Cataract affected Lens from 30 days old rat
Plate 4.7.D Protein Profile of Selenium Induced Cataract affected Lens After One Month old rat
5 DISCUSSION
5.1 Lens Weight
5.2 Oxidative stress
5.3 Lipid Peroxidation
5.4 Glycoprotein
5.5 Effect of VitaminE and Vitamin C on Cataract
5.6 Electrophoresis
6 SUMMARY AND CONCLUSION
REFERENCE