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TITLE
CERTIFICATE 1
CERTIFICATE-2
CERTIFICATE 3
DECLARATION
ACKNOWLEDGEMENT
CONTENTS
ABBREVIATIONS
1. INTRODUCTION AND REVIEW OF LITERATURE
1.1 Biosynthesis of Rubber from Sucrose
1.2. Factors influencing metabolism of laticifers
13. Factors related to mechanism of latex flow and production
1.4. Clonal variations in carbohydrate metabolism and pH balance
1.5. Factors involved in the latex regeneration mechanism in Hevea latex
1.6. Latex regeneration metabolism and isoprene synthesis
1.7. Phospholipids, glycolipids and latex production
1.8. Membrane enzymes involved in proton transport
1.9. Enzymes involved in latex production through coagulation
1.10. Carbohydrate metabolism and latex flow
2. MATERIALS AND METHODS
2.1. Location
2.2.1 Plant material for experiment
2.3 Latex Collection and seperation of Different Fractions for Biochemical Analysis
2.4 Dry Rubber Yield
2.5 DRC
2.6 Latex PH
2.7 C-and B-Serum PH
2.8 Amount of C-Serum, Rubber Cream and Bottom Fraction
2.9 Busting Index (BI) of Lutoids
2.10 Estimation of Thiols, Inorganic Phosphorous and Sucrose in Latex
2.11 Lipid Extraction from Latex, Rubber Cream and Bottom Fraction
2.12 Estimation of Phospholipids
2.13 Estimation of Glycolipid
2.14 Estimation of Sialic acid B Serum
2.15 Estimation of Protien Bound Hexose and Fucose in B-Serum
2.16 Estimation of Sucrose Synthase Activity in C-Serum
2.17 Estimation of Invertase Activity in C-Serum
2.18 ATP ase Activity of Lutoids
2.19 Pyrophosphatase Activity of C-Serum
2.20 Pyrophosphatase Activity of Lutoids
2.21 N-Acetyl Glucosaminidase in B-Serum
2.22 Chitinase Activity in B-Serum
2.23 Hevein Content of B-Serum
2.24 Estimation of Pyrophosphate
2.25 Estimation of total protein in C-and B-serum
2.26. Extraction of bark samples for biochemical analysis
3. RESULTS
3.1 Variations in Physiological and Biochemical Parameters Associated with Latex Production
3.2 Seasonal variations in carbohydrates and related enzymes associated with latex production in clone RRII 105 under 1/2s d/2 6d/7 system of tapping
3.3. Clonal variations in parameters associated with latex production
3.4. Physiological and biochemical parameters related to carbohydrate metabolism and ionic balance under 1/2 S d/2 6d/7and 1/2 S d/4 6d/7 tapping system in clone RRII 105
3.5. Effect of stimulation on carbohydrate metabolism and ionic balance in clone RRII 105 under 1/2S d/4 6d/7 system of Tapping
3.6. Biochemical parameters related to carbohydrate metabolism in trees affected by TPD
4. DISCUSSION
4.1. Seasonal variations in yield and other physiological parameters related to yield
4.2. Seasonal variation carbohydrates and related enzymes associated with latex production
4.3.Clonal variations in parameters associated with latex production
4.4. Parameters related to carbohydrate metabolism and ionic balance under d/2and d/4 system.
4.5. Effect of stimulation on different biochemical parameters (related tocarbohydrate metabolism and pH Regulation) in clone RRII 105 under d/4 system of tapping.
4.6. Changes in biochemical parameters related to carbohydrate metabolism in TPD affected trees
CONCLUSION
SUMMARY
REFERENCES