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TITLE
DEDICATION
CERTIFICATE
DECLARATION
ACKNOWLEDGEMENT
LIST OF ABBREVIATIONS
contents
1 Introduction
2 Review of literature
Fig 2.1: The Leloir Pathway.
Fig 2.2: The polyol Pathway
Fig 2.3: Oxidation of galactose.
Fig 2.4: Golactose metabolism in RBC of transferase deficient persons.
3 Materials and Methods
Experimental Grouping
I Experimental Group
II Control Group
Collection and preservation of blood
Preparation of lens extract
Chemicals
Table 3.1: Stability of enzymes and metabolic intermediates at 4°C (in days)
Table 3.2: Details of Instruments used for experiment.
Table 3.3: Stability of reagents
Preparation of hemolyzate
Estimation of Haemoglobin.
Estimation of Water Soluble, Insoluble and Total Protein.
Estimation of Glucose- 6- Phosphate dehydrogenase andGPhosphogluconate Dehydrogenase Activity.
Estimation of Glutathiorre Reductase.
Assay of Glutathione Peroxidase.
Estimation of Glutathione-s-Transferase.
Estimation of Catalase.
Estimation of Superoxide Dismutase.
Estimation of Transaldolase.
Estimation of Transketalase.
Estimation of Reduced Glutathione Level.
Measurement of Lipid Peroxidation.
Statistical Analysis
4. Results and Discussion
Fig.4.1 Various stages of cateractogenesis
Fig.4.2: Mean wet weight of lens (mg) in normal and catractout rats I during experiment
4A. Pentose pathway and Galactose metabolism
Table 4.A.1: Activity of G6PD in lens and erythrocytes (IU/qHb or Protein)
Table 4.A.2: Activity of 6PGD in lens and erythrocytes (IU/gHb or Protein)
Table 4.A.3: Activity of transaldolase in lens and erythrocytes (IUIqHb or Protein)
Table 4.A.4: Activity of transketofase in fens and erythrocytes (IU/qHb or Protein)
Fig. 4.4.1: HMP shunt and its relation to galactose metabolism
4B. The Glutathione System
Table 4.8.1: Activity of GR in lens ond erythrocytes (IUIsHb or Protein)
Table 4.8.2: G6PDlGR ratio in lens and erythrocytes.
Table 4.8.3: Activity of GSH-Px in lens and erythrocytes (IUlgHb or Protein)
Table 4.8.4: Reduced glutathione in lens and erythrocytes (WgHb or Protein)
Fig 4.B.1: The Glutethion cycle
Fig.4.B.2: Comparison of GR and GSM experimental rats
Fig.4.B.3: Comparison of GSH-Px and GSH in experimental rats
4C. Mercapturic acid Pathway and Lipid peroxidation
Table 4.C.1: Activity of GsT in lens and erythrocytes (IUIgHb or Protein)
Table 4.C.2: Malonaldehyde in lens and Plasma (nmolig Protein or id1 plasma)
Fig 4.C.3: Malonaldehyde and GsT in lens of experimental group
4D. The Antioxidant Enzymes
Table 4.D.I: Activity of catalase in lens and erythrocytes (x lW IUfg Hb or Protein)
Table 4.D.2: Activity of SOD in lens and erythrocytes (Ufg Hb or Protein)
Fig.4.D.1: Comparison of GSH-Px catalase and SOD in experimental rats
Fig.4.D.2: Schematic representation of H2O2 metabolism
4E. Lens Proteins
Table4.E.1: Total protein in lens and erythrocytes (mglg wet wt.)
Table 4.E.Z: Water soluble protein in lens (mg/g wet wt.)
Table 4.E.3: Water insoluble protein in lens (mg/g wet wt.)
Fig.4.E.1: Percentage of soluble-insoluble fractions in cataractous lens.
5.General Discussion
Table 5.1: Coefficient of correlation (r) between same enzymesof lens and erythrocytes or plasma.
Table 5.2: Coefficient of correlation (r) between various parmeters in lens
Table 5.3: Coefficient of correlation (r) between various parmetersin erythrocyte
Table 5.4: Coefficient of correlation (r) between lnsoluble proteinand various parameters
Fig.5.1: Schematic representation of results and its explanations
6. Summary
BIBILIOGRAPHY
APPENDIX