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TITLE
CERTIFICATE
DECLARATION
ACKNOWLEDGEMENT
CONTENTS
INTRODUCTION AND REVIEW
Cataracts
1. Developrental cataracts
2. Degenerative cataracts
senile cataract
1. Incipient stage
2. Intumescent stage
3. Mature stage
4. Hypermature stage
MATERIALS AND METHODS
Stages of cataract
(a) Immature cataract
(b) Mature cataract
(c) Hypermature cataract
Collection and transportation of samples
(a) Blood samples
(b) Lens samples
Preparation of freezing mixture
Preparation of haerolysate
Preparation of lens homogenate
Preparation of Drabkins solution
Preparation of soluble and insoluble protein fractions of lens
Determination of transketolase activity
Preparation of solutions
incubation
anthrone method
Orcinol lethod
Determination of ribose content
Assay of enzyme by macro method
Assay of glutathione reductase
Glutathione peroxidase
RESULTS
Blood sugar
Lens Protein
Glucose 6-phosphate dehydrogenase
Transketolase
Transaldolase
Glutathione reductase
Reduced glutathione
Correlation analysis
DISCUSSION
Incidence of Cataract
Blood Sugar
Lens Protein
Glucose 6-phosphate dehydrogenase
Transketolase
Transaldolase
Glutathione Reductase
Reduced Glutathione
Glutathione Peroxidase
SUMMARY AND CONCLUSION
BIBLIOGRAPHY