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  • TITLE
  • DEDICATION 1
  • DEDICATION 2
  • CERTIFICATE 1
  • CERTIFICATE-2
  • DECLARATION
  • ACKNOWLEDGEMENT
  • CONTENTS
  • LIST OF FIGURES
  • LIST OF PLATES
  • ABBREVIATIONS
  • I. INTRODUCTION
  • KERALA: LAKES, BACKWATERS AND RIVERS
  • AREA OF STUDY
  • RIVERS OF KERALA
  • LAKES AND BACKWATERS
  • The River Meenachil
  • II. REVIEW OF LITERATURE
  • PART I
  • II.1.1 Intestinal bacterial flora of avian fauna
  • II.1.2 Intestinal bacterial flora of Homo sapiens
  • PART II
  • II.2.1 Antibiotic sensitivity
  • II.3.1 Haemagglutination and haemolysis
  • II.4.1 Bacteriocin production
  • PART III
  • II.5.1 Effect of crop protection chemicals on microbial flora
  • II.6.1 Bacteriological studies on water
  • II.7.1 Survival ability of bacteria in aquatic environments
  • III. MATERIALS AND EXPERIMENTAL METHODS
  • PART I
  • III.1 Avian fauna subjected to investigation
  • III.1.1 Corvus splendens protegatus (Madarasz)
  • III.1.2 Phalacrocorax niger (Vieiilot)
  • III.1.3 Bubulcus ibis coromandus (Boddaert)
  • III.1.4 Anus crecca crecca (Linnaeus)
  • III.1.5 Anus domesticus (Domestic Duck)
  • III.1.6 Callus domesticus (Domestic Fowl)
  • III.1.7 Columba livia intermedia (Strickland)
  • Plate 1. Corvus splendens protegatus (Madarasz) The Common House Crow (Locality: Kottayam)
  • Plate 2. Corvus splendens protegatus (Madarasz) The Common House Crow (Locality: Ettumanoor)
  • Plate 3. Phalacrocorax niger (Vieillot) Little Cormorant (Locality: Kumarakom Tourist Complex)
  • Plate 4. Phalacrocorax niger (Vieillot) Cormorants and Darters (Locality: Kumarakom Tourist Complex)
  • Plate 5. Bubulcus ibis coromandus (Boddaert) Cattle Egret (Locality: Paddy fields, Athirampuzha)
  • Plate 6. Anas crecca crecca (Linnaeus) Common Teal (Locality: Kumarakom - Vembanad Lake)
  • Plate 7. Anas querquedula (Linnaeus) Garganey Teal (Locality: Kumarakom - Vembanad Lake)
  • Plate 8. Anas domesticus (Domestic Duck) (Locality: Athirampuzha)
  • Plate 9. Gallus domesticus (Domestic Fowl) (Locality: Poultry Farm, Kottayam)
  • Plate 10. Columba livia intermedia (Strickland) Indian Blue Rock Pigeon (Locality: Kottayam)
  • III.1.8 Collection of dropping pilings of birds
  • III.1.9 Isolation of bacteria from faecal samples of avian fauna
  • III.1.10 Identification of Bacterial Isolates
  • III.2 Antimicrobial Sensitivity Tests
  • III.2.1 Antimicrobial Drugs and Disc Potency Used for the Present Study
  • III.2.2 Stokes Method
  • III.3A Haemolysis
  • III.3B Haemagglutination Reaction
  • III.4 Test for Bacteriocin Production
  • III.5 Effect of Pesticides on Soil Microorganisms
  • III.6 Bacteriological Studies on Water Samples
  • III.7 Survival of Coliforms in Water Samples
  • IV. RESULTS
  • PART I
  • INTESTINAL GRAM NEGATIVE BACTERIAL FLORA OF AVIAN FAUNA
  • IV. 1.1 Incidence of bacterial species from avian faecal sources
  • Fig. 1 Percentage occurrence of Gram negative intestinal bacteria of avian fauna
  • Fig. 2. Percentage occurrence of Gram negative intestinal bacteria of Crow, Egret, Duck, and Cormorant
  • Fig. 3. Percentage occurrence of Gram negative intestinal bacteria of Teal, Pigeon, Fowl, and Cormorant
  • Plate 11. Cormorant: Direct smear from droppings (Gram stained)
  • Plate 12. Cormorant: Sweep smear from culture (Gram stained)
  • Plate 13. Crow: Sweep smear from culture (Gram stained)
  • Plate 14. Egret: Sweep smear from culture (Gram stained)
  • Plate 15. Teal: Direct smear from droppings (Gram stained)
  • Plate 16. Teal: Sweep smear from culture (Gram stained)
  • Plate 17. Duck: Sweep smear from culture (Gram stained)
  • Plate 18. Fowl: Sweep smear from culture (Gram stained)
  • Plate 19. Pigeon: Direct smear from droppings (Gram stained)
  • Plate 20. Pigeon: Sweep smear from culture (Gram stained)
  • PART II
  • Characterisation
  • IV.2.1 Antimicrobial susceptibility profiles of bacterial isolates from human and avian sources
  • IV.2.2 Multiple drug resistance of bacterial isolates from human and avian sources
  • Fig. 4. Antibiotic susceptibility profiles of E. coli from avian and human sources
  • Fig. 5. Antibiotic susceptibility profiles of Klebsiella sp. from avian and human sources.
  • IV.3A.1 Haemolytic property of the human and avian Gram negative bacterial isolates
  • IV. 3B.1 Haemagglutination activity of the human and avian Gram negative bacterial isolates
  • IV.4.1 Bacteriocin production profile of Gram negative enteric bacteria from human and avian sources
  • Fig. 6. Incidence of drug resistance in bacteriocinogenic and nonbacteriocinogenic strains - avian source
  • Fig. 7. Incidence of drug resistance in bacteriocinogenic and nonbacteriocinogenic strains - human source
  • Plate 21. Bacteriocin production (Negative.)
  • Plate 22. Bacteriocin production (Positive)
  • PART III
  • COLIFORMS AND OTHER ENVIRONMENTAL BACTERIAL FLORA
  • I V.5.1 Effect of crop protection chemicals on bacterial flora of the soil
  • IV.6.1 Bacteriological studies on water-prevalence of coli forms
  • IV.6.2 Eijkman test and faecal coliforms
  • 1V.6.3 Incidence of Gram positive bacteria in various aquatic environmtents
  • IV.6.4 Incidence of NLF bacteria in various aquatic environments
  • IV.6.5 E. coli count and environmental parameters
  • Fig. 8. Seasonal variations of bacteria in well water
  • Fig. 9. Seasonal variations of bacteria in pond water
  • Fig. 10. Seasonal variations of bacteria in paddy field water
  • Fig. 11. Seasonal variations of bacteria in streams
  • Fig. 12. Seasonal variations of bacteria in river water
  • IV.7.1 Survival ability of coliforms and Gram negative pathogenic enteric bacteria in various aquatic environments
  • Fig. 13. Average survival ability of bacteria in various aquatic environments
  • Fig. 14. Relationship between survival rate and salinity percentage of E. coli in freshwaters.
  • Fig. 15. Relationship between survival rate and organic content of E. coli in freshwaters
  • V. DISCUSSION
  • PART I
  • V.1.1 Prevalence of bacterial species from avian faecal sources.
  • Plate 23. Himantopus himantopus himantopus (Blackwinged stilt) (Locality: Athirampuzha)
  • Plate 24. Kumarakom backwaters
  • PART II
  • V.2.1 Antimicrobial susceptibility profiles
  • V.3A.1 Haemolysin production activity of bacterial isolates from human and avian sources
  • V.3B.1 Haemagglutination activity of the human and avian isolates.
  • V.4.1 Bacteriocin production profile of Gram negative enteric bacteria from human and avian sources
  • PART III
  • V.5.1 Effect of pesticides on microbial flora of the environment.
  • V.6.1 Bacteriological studies on samples from freshwater environments
  • V.7.1 Survival ability of Gram negative bacteria in aquatic environments
  • VI. SUMMARY AND CONCLUSION
  • BIBLIOGRAPHY
  • APPENDIX