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Full Screen- TITLE
- DECLARATION
- CERTIFICATE
- DEDICATION
- ACKNOWLEDGEMENT
- ABSTRACT
- PREFACE
- CONTENTS
- LIST OF TABLES
- LIST OF FIGURES
- ABBREVIATIONS
- 1. INTRODUCTION
- CONTENTS
- I. The Genus Psophocarpus Necker ex. A. P. de Candolle
- II. Reproductive biology
- III. Tissue culture
- IV. Micropropagation of grain legumes
- V. Variations in tissue culture
- VI. Scope of the work
- 2. REVIEW OF LITERATURE
- 3. MATERIALS AND METHODS
- CONTENTS
- I. Reproductive biology
- I. 1. Morphology and ultra-structure of reproductive parts
- I.2. Pollen viability
- I. 3. General histochemistry and enzyme cytochemistry
- II. Seed germination studies
- III. In vitro techniques for plant propagation
- III.1. Initiation of tissue culture
- III. 2. Washing and sterilization of glassware and appliances
- III. 3. Media preparation
- III. 4. Experimental protocols
- III. 5. Rooting
- III. 6. Hardening and field establishment of plantlets
- III. 7. Statistical analysis
- IV. Biochemical analysis of callus
- IV. 1. Callus Cytochemistry
- IV. 2. Enzyme Cytochemistry
- IV. 3. Estimation of proteins
- IV. 4. Estimation of chlorophyll pigments
- V. Analysis of Variation
- V. 1. Random Amplified Polymorphic DNA (RAPD)
- V.2. Protein profile
- 4. RESULTS
- CONTENTS
- I. Reproductive biology
- I.1. Description of the plant
- I.2. Morphology and ultrastructure of the reproductive organs
- II. Seed storage and germination studies
- III. Tissue culture studies
- III.1. Sterilization of explants
- III. 2. Axillary bud proliferation
- III. 3. De novo organogenesis
- III. 4. Indirect organogenesis
- III. 5. Somatic embryogenesis
- III. 6. Rooting of shoots
- III. 7. Hardening and acclimatization
- IV. Callus cytochemistry and histochemistry
- IV.1. Callus cytochemistry
- IV.2. Enzyme histochemistry
- IV.3. Biochemistry of the organogenic callus
- V. Analysis of variation
- V. 1. Random Amplified Polymorphic DNA (RAPD) Analysis
- V. 2. Protein profile
- TABLES
- List of Tables
- FIGURES
- List of Figures
- PLATE 1
- PLATE 2
- PLATE 3
- PLATE 4
- PLATE 5
- PLATE 6
- PLATE 7
- PLATE 8
- PLATE 9
- PLATE 10
- PLATE 11
- PLATE 12
- PLATE 13
- PLATE 14
- PLATE 15
- PLATE 16
- PLATE 17
- PLATE 18
- PLATE 19
- PLATE 20
- PLATE21
- PLATE22
- PLATE23
- 5. DISCUSSION
- CONTENTS
- I. Reproductive Biology
- I. 1. Morphology and ultrastructure of reproductive organs
- I. 2. General histochemistry and enzyme cytochemistry
- II. Seed germination
- III. Tissue culture studies
- III. 1. Selection of medium
- III. 2. Shoot tip, cotyledonary node and nodal segment culture
- III. 3. Elongation of shoots
- III. 4. Direct Regeneration
- III. 5. Callus induction
- III. 6. Callus regeneration
- III. 7. Somatic embryogenesis
- III. 8. Rooting and Hardening of Shoots
- IV. Callus Cytochemistry and histochemistry
- IV. 1. Callus cytochemistry
- IV. 2. Enzyme histochemistry
- IV. 3. Estimation of protein
- IV. 4. Estimation of Chlorophyll pigments
- V. Analysis of variation
- V. 1. RAPD
- V. 2. Protein Profile
- 6. SUMMARY AND CONCLUSION
- BIBLIOGRAPHY