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Full Screen- Title
- DECLARATION
- CERTIFICATE
- ACKNOWLEDGEMENT
- contents
- Part I Introduction (Review of literature) ORAL CANCER
- 1.1 Natural history
- Incidence and prevalence
- Etiology
- 1.2 Diagnosis of oral cancer
- Histopathological staging of oral cancer
- Clinical staging
- 1.3 Management of oral cancer
- Tumor kinetics
- Chemotherapy
- Radiotherapy
- Treatment planning
- 1.4 Molecular basis of oncogeneic transformation in oral cancer
- p53 Structural Details and Mutational Spectrum
- Apoptosis (Programmed Cell Death)
- Historical perspectives of apoptosis
- Significance of apoptosis in cancer
- Radiation and chemotherapy Induces apoptosis
- Apoptotic machinery and mechanisms
- The genetic regulation of apoptosis
- Role of p53, Bcl-2 group of proteins
- 1.5 Tumor angiogenesis.
- 1.6 Tissue proliferative compartment.
- 1.7 p53, apoptosis and angiogenesis.
- Part II Aims and objectives
- 2.1 Background and justification of the problem
- 2.2 Factors influencing treatment response
- 2.3 Plan of the study
- 2.4 Aims
- 2.5 Specific objectives
- Part III Materials and methods.
- 3.1 Study subjects and tissue preparation
- Radiotherapy Schedule
- 3.2 TUNEL
- 3.3 Immunocytochemistry
- Evaluation of immunocytochemistry results.
- 3.4 Details of antibodies used in the study
- 3.5 ELISA for mutant p53 protein
- Protein extraction
- 3.6 Agyrophilic Nuclear Organizers
- 3.7 Justification for methodologies
- 3.8 Statistical analysis and review of statistics
- Part IV Tumor progression: Role of apoptosis, its regulation, angiogenesis and proliferation during pathobiological progression.
- 4.1 Introduction
- 4.2 Results
- 4.3 Correlation analysis for the association of biological parameters with histological progression of the disease
- 4.4 p53, apoptosis and apoptosis regulation
- 4.5 Tumor angiogenesis
- 4.6 Discussion
- Influence of angiogenesis on apoptosis and proliferation
- 4.7 Conclusion
- Part V Prognostic significance of p53 expression, apoptosis, apoptotic regulatory proteins, angiogenesis and proliferation in invasive lesions.
- 5.1 Introduction
- 5.2 Study subjects and radiotherapy schedule
- 5.3 Results
- Treatment response
- Correlation among the experimental parameters in invasive lesions
- Correlation with clinical parameters
- Mutant p53 protein
- Multiple regression analysis
- 5.4 Discussion
- 5.5 Conclusion
- Part VI Design of a predictive assay.
- 6.1 Introduction
- 6.2 Hypothesis
- 6.3 Preliminary design and investigations
- 6.4 Results
- 6.5 Conclusion
- Part VII Conclusion
- Applications of the results in disease management and future prospects for research.
- Part VIII Summary of the study
- Summary of results
- CONCLUSION
- APPENDIX
- List of references and publications.