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TITLE
DEDICATION
CERTIFICATE-1
CERTIFICATE-2
DECLARATION
ACKNOWLEDGEMENT
CONTENTS
LIST OF TABLES
LIST OF FIGURES
LIST OF PLATES
ABBREVIATIONS
1. Introduction
1.1 Preface
1.2 A glimpse into the previous studies
1.2.1 Effect of light on seed germination
1.2.1.1. Interaction between light and other factors
1.2.2 Spectral analysis and discovery of phytochrome
1.2.3 Light induced changes in metabolism
1.2.4 Photo regulation of enzymes
1.1 Index of enzymes reported to be controlled by phytochrome
1.2.4.1 Mechanism of enzyme regulation by light
1.2.4.1.1 Regulation by gene activation
1.2.4.1.2 Activation of enzymes by light
1.2.4.1.2.1 Mechanisms of light activations
1.2.5 Phytochrome-An unfinished agenda
1.2.5.1 Multiplicity of phytochromes
1.2.5.2. Structure of phytochrome
1.2.5.3. Signal transduction through phytochrome
1.2.5.3.1. Change in membrane permeability and phosphorylation of proteins
1.2.5.3.2 Regulation of gene expression
1.2 Light regulated nuclear genes in higher plants
1.2.5.4. Central dogma of phytochrome action
1.2.6. Blue light effects and blue light receptors
1.2.6.1. Blue light receptors
1.2.6.2. Mechanism of action of blue light
1.3. Objectives of present study
2. Influence of light on proteins and proteases
2.1. Introduction
2.2 Review of literature
2.3 Materials and methods
2.3.1 Experimental plants
2.3.2 Growth conditions
2.3.3 Light treatments
2.3.4 Estimation of total soluble proteins
2.3.5 SDS - PAGE
2.3.6 Determination of total protease activity
2.4 Results
2.1 Total soluble protein content in Vigna unguiculata seedlings grown under different-wavelength-lights with initial light phase
2.1 Total soluble protein content in Vigna unguiculata seedlings grown under different-wavelength-lights with initial light phase
2.2 Total soluble protein content in Arachis hypogaea seedlings grown under different-wavelength-lights with initial light phase
2.2 Total soluble protein content in Arachis hypogaea seedlings grown under different-wavelength-lights with initial light phase
2.3 Total soluble protein content in Oryza sativa seedlings grown under different-wavelength-lights with initial light phase
2.3 Total soluble protein content in Oryza sativa seedlings grown under different-wavelength-lights with initial light phase
2.4 Total soluble protein content in Vigna unguiculata seedlings grown under different-wavelength-lights with initial dark phase
2.4 Total soluble protein content in Vigna unguiculata seedlings grown under different-wavelength-lights with initial dark phase
Plate 2.1 Protein profile of Vigna unguiculata seedlings grown under different-wavelength-lights with initial light phase (First day)
2.5 Protein profile of Vigna unguiculata seedlings grown under different-wavelength-lights with initial light phase (First day)
Plate 2.2 Protein profile of Vigna unguiculata seedlings grown under different-wavelength-lights with initial light phase (Second day)
2.6 Protein profile of Vigna unguiculata seedlings grown under different-wavelength-lights with initial light phase (Second day)
Plate 2.3 Protein profile of Vigna unguiculata seedlings grown under different-wavelength-lights with initial light phase (Third day)
2.7 Protein profile of Vigna unguiculata seedlings grown under different-wavelength-lights with initial light phase (Third day)
Plate 2.4 Protein profile of Vigna unguiculata seedlings grown under different-wavelength-lights with initial light phase (Fourth day)
2.8 Protein profile of Vigna unguiculata seedlings grown under different-wavelength-lights with initial light phase (Fourth day)
Plate 2.5 Protein profile of Vigna unguiculata seedlings grown under different-wavelength-lights with initial dark phase (First day)
2.9 Protein profile of Vigna unguiculata seedlings grown under different-wavelength-lights with initial dark phase (First day)
Plate 2.6 Protein profile of Vigna unguiculata seedlings grown under different-wavelength-lights with initial dark phase (Second day)
2.10 Protein profile of Vigna unguiculata seedlings grown under different-wavelength-lights with initial dark phase (Second day)
Plate 2.7 Protein profile of Vigna unguiculata seedlings grown under different-wavelength-lights with initial dark phase (Third day)
2.11 Protein profile of Vigna unguiculata seedlings grown under different- wavelength-lights with initial dark phase (Third day)
Plate 2.8 Protein profile of Vigna unguiculata seedlings grown under different-wavelength-lights with initial dark phase (Fourth day)
2.12 Protein profile of Vigna unguiculata seedlings grown under different- wavelength-lights with initial dark phase (Fourth day)
2.13 Total protease activity in Vigna unguiculata seedlings grown under different-wavelength-lights with initial light phase
2.5 Total protease activity in Vigna unguiculata seedlings grown under different-wavelength-lights with initial light phase
2.14 Total protease activity in Arachis hypogaea seedlings grown under different-wavelength-lights with initial light phase
2.6 Total protease activity in Arachis hypogaea seedlings grown under different-wavelength-lights with initial light phase
2.15 Total protease activity in Oryza sativa seedlings grown under different-wavelength-lights with initial light phase
2.7 Total protease activity in Oryza sativa seedlings grown under different-wavelength-lights with initial light phase
2.16 Total protease activity in Vigna unguiculata seedlings grown under different-wavelength-lights with initial dark phase
2.8 Total protease activity in Vigna unguiculata seedlings grown under different-wavelength-lights with initial dark phase
2.5 Discussion
3. Influence of light on lipase
3.1 Introduction
3.2 Review of literature
3.3 Materials and methods
3.3.1 Experimental plants
3.3.2 Growth conditions
3.3.3 Light treatments
3.3.4 Determination of lipase activity
3.4 Results
3.1 Lipase activity in Vigna unguiculata seedlings grown under different-wavelength-lights with initial light phase
3.1 Lipase activity in Vigna unguiculata seedlings grown under different- wavelength-lights with initial light phase
3.2 Lipase activity in Arachis hypogaea seedlings grown under different- wavelength-lights with initial light phase
3.2 Lipase activity in Arachis hypogaea seedlings grown under different- wavelength-lights with initial light phase
3.3 Lipase activity in Oryza sativa seedlings grown under different- wavelength-lights with initial light phase
3.3 Lipase activity in Oryza sativa seedlings grown under different-wavelength-lights with initial light phase
3.4 Lipase activity in Oryza sativa seedlings grown under different- wavelength-lights with initial dark phase
3.4 Lipase activity in Oryza sativa seedlings grown under different-wavelength-lights with initial dark phase
3.5 Average Lipase activity for seven days of Vigna unguiculata seedlings grown under different-wavelength-lights with initial light phase
3.6 Average Lipase activity for seven days of Arachis hypogaea seedlings grown under different-wavelength-lights with initial light phase
3.7 Average Lipase activity for seven days of Oryza sativa seedlings grown under different-wavelength-lights with initial light phase
3.8 Average Lipase activity for seven days of Oryza sativa seedlings grown under different-wavelength-lights with initial dark phase
3.5 Discussion
4. Influence of light on amylases
4.1 Introduction
4.2 Review of literature
4.3 Materials and methods
4.3.1 Experimental plants
4.3.2 Growth conditions
4.3.3 Light treatments
4.A Different types of red light irradiation
4.3.4 Determination of amylase activity
4.4 Results
4.1 α-Amylase activity in Vigna unguiculata seedlings grown under different- wavelength-lights with initial light phase
4.1 α-Amylase activity in Vigna unguiculata seedlings grown under different-wavelength-lights with initial light phase
4.2 α -Amylase activity in Arachis hypogaea seedlings grown under different-wavelength-lights with initial light phase
4.2 α -Amylase activity in Arachis hypogaea seedlings grown under different-wavelength-lights with initial light phase
4.3 α -Amylase activity in Oryza sativa seedlings grown under different-wavelength-lights with initial light phase
4.3 α -Amylase activity in Oryza sativa seedlings grown under different- wavelength-lights with initial light phase
4.4 α -Amylase activity in Oryza sativa seedlings grown under different-wavelength-lights with initial dark phase
4.4 α -Amylase activity in Oryza sativa seedlings grown under different- wavelength-lights with initial dark phase
4.5 α -Amylase activity in Oryza sativa seedlings grown under different-wavelength-lights with initial light phase at 6 hrs interval
4.5 α -Amylase activity in Oryza sativa seedlings grown under different- wavelength-lights with initial light phase at 6 hrs interval
4.6 α -Amylase activity in Oryza sativa seedlings grown under different-wavelength-lights with initial dark phase at 6 hrs interval
4.6 α -Amylase activity in Oryza sativa seedlings grown under different- wavelength-lights with initial dark phase at 6 hrs interval
4.7 α -Amylase activity of 72 hrs old Oryza saliva seedlings undergone different durations of red light treatment
4.7 α -Amylase activity of 72 hrs old Oryza sativa seedlings undergone different durations of red light treatment
4.8 α -Amylase activity of 96 hrs old Oryza saliva seedlings undergone different durations of red light treatment
4.8 α -Amylase activity of 96 hrs old Oryza sativa seedlings undergone different durations of red light treatment
4.9 α -Amylase activity of 120 hrs old Oryza saliva seedlings undergone different durations of red light treatment
4.9 α -Amylase activity of 120 hrs old Oryza sativa seedlings undergone different durations of red light treatment
4.10 β-Amylase activity in Vigna unguiculata seedlings grown under different-wavelength-lights with initial light phase
4.10 β-Amylase activity in Vigna unguiculata seedlings grown under different- wavelength-lights with initial light phase
4.11 β -Amylase activity in Arachis hypogaea seedlings grown under different-wavelength-lights with initial light phase
4.11 β -Amylase activity in Arachis hypogaea seedlings grown under different- wavelength-lights with initial light phase
4.12 β -Amylase activity in Oryza saliva seedlings grown under different-wavelength-lights with initial light phase
4.12 β -Amylase activity in Oryza sativa seedlings grown under different-wavelength-lights with initial light phase
4.13 β -Amylase activity in Oryza saliva seedlings grown under different-wavelength-lights with initial dark phase
4.13 β -Amylase activity in Oryza sativa seedlings grown under different-wavelength-lights with initial dark phase
4.14 Average β -amylase activity for seven days of Vigna unguiculata seedlings grown under different-wavelength-lights with initial light phase
4.15 Average β -amylase activity for seven days of Arachis hypogaea seedlings grown under different-wavelength-lights with initial light phase
4.16 Average β -amylase activity for seven days of Oryza sativa seedlings grown under different-wavelength-lights with initial light phase
4.17 Average β -amylase activity for seven days of Oryza sativa seedlings grown under different-wavelength-lights with initial dark phase
4.5 Discussion
CONCLUSION
Summary
References