Online Thesis Search Results

TITLE
DEDICATION
CERTIFICATE
DECLARATION
ACKNOWLEDGEMENT
CONTENTS
ABBREVIATIONS
LIST OF FIGURES
LIST OF PLATES
LIST OF TABLES
1. INTRODUCTION
1. INTRODUCTION
1.1 Biodegradation
1 Ortho and Meta Pathway for the degradation of aromatic compounds
1.2 Review of literature
1 Microorganisms in the biodegradation of organic compounds
2 Microorganisms in the Biodegradation of phenolic compounds
3 Enzymes in the biodegradation of phenolic compounds
4 Biological treatment of phenolic wastewater
2. MATERIALS AND METHODS
2.1. Isolation and selection of a phenol degrading strain by enrichment culture
2.2. Identification of the selected bacterial strains
2.3. Optimization of the conditions of phenol degradation
2.4. Analysis of the products of phenol degradation
2.5. Isolation and purification of polyphenol oxidase from Alcaligenes sp d2
2.6. Treatment of the phenolic paper factory effluent
Plate 1 First Reactor
Plate 2 Second Reactor
Plate 3 Third Reactor
Plate 4 The Bottom Space for FAC Packing in the Reactor
Plate 5 The filter Bed of the Reactor for Packing Immobilized Cells.
Plate 6 The Space for Coating Chitosan in the Reactor
Plate 7 Chitosan Coating in the Reactor
Plate 8 Treatment of the Effluent with PAC Packed in the Reactor.
Plate 9 Treatment of the Effluent with Immobilized Cells
Plate 10 Treatment of the Effluent with Chitosan Coating
Plate 11 Treatment of the Effluent with the Three Stage Reactor.
3. RESULTS
3.1 Isolation, identification and selection of an efficient phenol degrading bacteria
5 Isolation of phenol tolerating strains by soil enrichment technique
6 Isolation of phenol degrading strains by enrichment technique using mineral salt phenol medium
3.2 Optimization of the conditions of biodegradation of phenol
7 Identification and selection of potential strain for the biodegradation of phenol
2 Effect of phenol concentration or. specific growth rate of Alcaligenes sp d2
3 Effect of substrate concentration on phenol degradation by Alcaligenes sp d2
4 Optimization of the incubation period for the biodegradation of phenol by Alcaligenes sp d2
5 Optimization of pH for the biodegradation of phenol by Alcaligenes sp d2
6 Growth curve of free cells of Alcaligenes sp d2 in mineral salt phenol medium (MSPM)
7 Growth curve of immobilized Alcaligenes sp d2 in mineral salt phenol medium (MSPM)
3.3 Analysis of the products of phenol biodegradation
8 Phenol degradation in mineral salt phenol medium (MSPM) by immobilized cells of Alcaligenes sp d2 in batch process
9 Gas chromatogram of the extract of uninoculated MSPM (control)
10 Mass spectroscopic analysis of the peak at 14.997 min in the gas chromatogram of the extract of uninoculated MSPM
11 Analysis of the extract of biodegraded phenol MSPM by Gas chromatography
12 Mass spectroscopic analysis of the additional peak at 23.394 min in the gas chromatogram of the extract of biodegraded MSPM
13 Mass spectroscopic analysis of the additional peak at 26.567 min in the gas chromatogram of the extract of biodegraded MSPM
14 Mass spectroscopic analysis of the additional peak at 36.204 min in the gas chromatogram of the extract of biodegraded MSPM
15 Mass spectroscopic analysis of the additional peak at 36.783m in. in the gas chromatogram of the extract of biodegraded MSPM
16 Mass spectroscopic analysis of the additional peak at 37.922 min in the gas chromatogram of the extract of biodegraded MSPM
17 Mass spectroscopic analysis of the additional peak at 38.988 min in the gas chromatogram of the extract of biodegraded MSPM
18 Mass spectroscopic analysis of the additional peak at 39.084 min in the gas chromatogram of the extract of biodegraded MSPM
19 Analysis of the extract of uninoculated MSPM by FT-IR
20 Library search report of the FT-IR analysis of the extract of uninoculated MSPM
21 FT- IR analysis of the extract of biodegraded MSPM
3.4 Polyphenol oxidase from Alcaligenes sp d2
22 Library search report of the FT-IR analysis of the extract of biodegraded MSPM
Plate 12 Native Page of the partially Purified Enzyme
8 Purification profile of polyphenol oxidase isolated from phenol degrading Alcaligenes sp d2
23 Effect of substrate (ARTS) on the activity of polyphenol oxidase from Alcaligenes sp d2
24 Line Weaver Burk Plot of Polyphenol oxidase from Alcaligenes sp d2 with ABTS as the substrate
25 Effect of pH on the activity of polyphenol oxidase from the Alcaligenes sp.d2
3.5 Application of the selected phenol degrading organism Alcaligenes sp d2 in waste water treatment
26 Biodegradation of phenol with partially purified polyphenol oxidase from Alcaligenes sp d2
9 Characteristics of the phenolic paper factory wastewater
27 Growth curve of free cells of Alcaligenes sp d2 in phenolic effluent
28 Treatment of phenolic effluent with free cells of Alcaligenes sp d2 in batch process
29 Growth curve of immobilized Alcaligenes sp d2 in phenolic effluent
30 Treatment of phenolic effluent with immobilized cells of Alcaligenes sp d2 in batch process
31 Phenol degradation and COD reduction in effluent by immobilized cells of Alcaligenes sp d2 by continuous treatment in a packed bed reactor
32 Performance of the packed bed reactor with immobilized cells of Aicakgenes sp d2 in the treatment of phenolic effluent by continuous process
33 Scanning of the coloured paper factory effluent for the absorption maxima by spectrophotometric analysis
34 Continuous treatment of the phenol paper factory effluent with powdered activated charcoal (PAC) packed in the reactor
35 Continuous treatment of phenolic paper factory effluent using immobilized cells of AIcaligenes sp d2 packed in the reactor
36 Continuous treatment of the phenol paper factory effluent using chitosan coated reactor
37 Continuous treatment of the phonolic paper factory effluent using the three stage activated charcoal; immobilized cell and chitosan packed reactors in series
4. DISCUSSION
4.1 Isolation identification and selection of an efficient phenol degrading bacteria
4.2 Optimization of the conditions of biodegradation
4.3 Analysis of the products of phenol biodegradation
4.4 Polyphenol oxidase from Alcaligenes sp d2
4.5 Application of the selected phenol degrading organism in waste water treatment
5. SUMMARY
6. CONCLUSION
REFERENCES
APPENDICES
APPENDIX I. Carbohydrate Sugar Fermentation Medium
APPENDIX II. Hugh-Liefson Agar Medium (O.F. Medium)
APPENDIX Ill. Methyl redNoges-Proskeaur Medium (MR-VP Medium)
APPENDIX IV. Methyl Red Solution
APPENDIX V. Nutrient agar Medium
APPENDIX VI. Nutrient Broth
APPENDIX VII. Oxidase Reagent
APPENDIX VIII. Phenol Red Dextrose Both
APPENDIX IX. Sulphide lndole Motility (SIM) Agar Medium
APPENDIX X. Simmons Citrate Agar Medium
APPENDIX XI. Starch agar medium
APPENDIX XII. Triple Sugar Iron Agar Medium
APPENDIX xIII. Tryptone Broth Medium
APPENDIX XIV. Gelatin Agar Medium
APPENDIX XV. Test solution for gelatin hydrolysis
APPENDIX XVI ANALYSIS REPORT
APPENDIX XVll ANALYSIS REPORT