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Title
DEDICATION
CERTIFICATE 1
CERTIFICATE-2
DECLARATION
ACKNOWLEDGEMENT
CONTENTS
List of Abbreviations
1 Introduction
Aims and objectives of the study
2 Review of Literature
2.1 Storage of Fishes
2.2 Storage of Fruits and Vegetables
2.3 Storage of Fishery Products
2.4 Storage of Meat Samples
3 Materials and Methods
3.1 Food Samples
3.2 Spices
3.3 Chemicals
3.4 Experimental Design
3.4.1 Storage Durations
3.4.2 Biochemical Analysis
Determination of Moisture Content (MC)
Estimation of Protein
Extraction of Tissue for Water Soluble Protein
Extraction of Tissue for Salt Soluble Protein
Estimation of Crude Protein
Extraction of Tissue for Lipid Estimation
Estimation of 1-opal Lipids
Estimation of Phospholipids
Estimation of Triglycerides (TG)
Estimation of Free Fatty Acids
Preparation of tissue homogenate for the estimation of lipid peroxides
Estimation of Thiobarbituric Acid Reactive Substances (TBARS)
Estimation of Conjugated Dienes (CD)
Estimation of Superoxide Dismutase (SOD)
Estimation of Glutathione
Estimation of Lactate Dehydrogenase (LDH)
Estimation of Glycogen
3.4.3 Statistical Analysis
3.4.4 Antimicrobial property of spices on refrigerated buffalo meat
4 Biochemical Changes of Food Samples During Frozen Storage
4.1 Introduction
4.2 Materials and Methods
4.2.1 Materials
4.2.2 Processirrg
I. Marine fishes
II.Fresh water fishes
III. Molluscs
IV. Meat samples
4.3 Results and Discussion
4.3.1 Effect of froden storage on proteins of food samples
4.3.2 Lipid profile during frozen storage
4.3.3 Changes in lipid peroxides
4.3.4 Changes of moisture content (MC), glycogen and lactate dehydrogenase (LDH)
5 Antimicrobial Effect of Plant Extracts on Minced Buffalo Meat
5.1 Introduction
5.2 Materials and Methods
5.2.1 Materials
1. ALL SPICE or PIMENTA
2. CORIANDER
3. CURRY LEAF
4. PEPPERMINT
5.2.2 Methods
1. Preparation of leaf extracts
2. Preparation of minced meat
3. Incubation of buffalo Ineat samples in plant extracts
5.3 Results and Discussion
5.3.1 Antimicrobial Properties of Food Additives on Minced Buffalo Meat underChilled Temperature
5.3.2 Antimicrobial Properties of Food Additives on Minced Buffalo Meatunder Frozen Temperature
6 General Discussion
Significance of the present investigation
7 Summary
References