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Full Screen- Title
- CERTIFICATE
- DECLARATION
- ACKNOWLEDGEMENT
- CONTENTS
- ABBREVIATIONS
- 1 Introduction and review of Literature
- INTRODUCTION
- Risk factors of atherosclerotic vascular disease
- 1. Hypercholesterolaemia
- Fig 1: Paracrine and autocrine interactions in atherosclerosis
- 2. Triglycerides
- 3. Lipoprotein (a)
- 4. Blood pressure
- 5. Epidemiological reasons
- 6. Cigarette smoking
- 7. Free radicals
- Fig 2: Schematic representation for oxidative stress induced tissue injury
- 8. Diabetes mellitus
- 9. Coagulation system
- 10. Platelet aggregation
- 11. Other factors
- a. Homocysteine and coronary risk
- Therapy of Atherosclerotic vascular disease
- Drugs
- 1. Hydroxy methyl glutaryl Co A reductase inhibitors
- 2. Bile acid binding resins
- 3. Gemfibrozil
- 4. Niacin
- Animal model
- 2 Materials and Methods
- 2.1 MATERIALS
- 2.1.1 Porcine Pancreas Tissue
- 2.1.2 Plant materials
- 2.1.3 Chemicals
- 2.1.4 Instruments
- 2.1.5. Animals
- 2.1.6 Maintenance of Animals
- 2.1.7 Test Compounds
- 2.1.8 Preparation of test materials
- 2.1.8a Isolation and characterisation of proteoglycans
- Nuclease Digestion
- Ion exchange chromatography
- Gel filtration of proteoglycans
- Alkali - Borohydrate digestion
- HNO, digestion
- Electrophoresis of HNO, digest
- 2.1.8 b Plant extracts
- 2.1.9 Antioxidant studies
- 2.1.9a Superoxide radical production by photo reduction of riboflavin (204)
- 2.1.9b Hydroxyl radical generation (205)
- 2.1.9c Lipid peroxide formation (206)
- 2.1.10 Lipoprotein lipase releasing activity
- 2.1.11 Anticoagulant studies
- 2.1.11a Plasma recalcification time
- 2.1.11 b Activated partial thromboplastin time test
- 2.1.12 Platelet aggregation studies (208)
- 2.1.13 Platelet arachidonate pathway (209)
- 2.1.14 Antiinflammatory studies
- 2.1.15 Anti-atherogenic study
- Aortal staining for fat deposition (214)
- 2.1.16 Statistical evaluation
- 3 Isolation and characterisation of proteoglycans from Porcine Pancreas
- Introduction
- Materials and methods
- Results
- Fig. 3.1: DE 52 Ion Exchange Chromato Graphy of Guanidine Extracted materials fromPorcine Pancreas
- Fig. 3.11: Gel filtration of Proteoglycan fraction A on Sepharose 6B column
- Fig. 3.111: Gel filtration of Proteoglycan fraction B on Sepharose 6B column
- Fig. 3.IV: Gel filtration of Proteoglycan fraction C on Sepharose 6B column
- Plate 3-1: Agarose gel electrophoresis of Glycosamlnoglycan moiety of PG A fraction
- DISCUSSION
- 4 Antioxidant and anti-inflammatory effect of some plant extracts
- Introduction
- Materials and methods
- Superoxide scavenging activity
- Hydroxyl radical scavenging activity
- Lipid peroxide formation in vitro
- Anti-inflammatory activity in vivo
- Results
- Anti-inflammatory effect of R. damascena
- DISCUSSION
- 5 Effect of proteoglycans and some plant extracts on lipoprotein lipase activity
- Introduction
- Materials and methods
- DISCUSSION
- 6 Antithrombotic effect of proteoglycans and some plant extracts
- Introduction
- Materials and methods
- Anticoagulant activity
- Plasma recalcification method
- Activated prothrombin time method
- Platelet aggregation studies in vitro
- Study on platelet arachidonate path way in vitro
- Anti platelet aggregatory activity
- Effect on arachidonic acid metabolism
- DISCUSSION
- 7 Antiatherogenic effect of proteoglycan and acetone fraction of R.damascena
- Introduction
- Materials and Methods
- Results
- Fig. 7.I: Percentage increase in the serum lipid profile of control and treated groups compared tonormal groups one month after experiment.
- Fig. 7. II: Percentage increase in the serum lipidprofile of control and treated groups comparedto normal groups two months after experiment.
- Fig. 7.III: Percentage increase in the serum lipid profile of control and treated groups comparedto normal groups three months after experiment
- Fig. 7.IV: Percentage increase in the serum lipid profile of control and treated groups comparedto normal groups four months after experiment.
- DISCUSSION
- Plate 7-1: Aorta of control group animals after oil red 0 stalning showing areas offat deposition
- Plate 7-11: Aorta of AF treated group animals after oil red 0 staining showingareas of fat deposition
- Plate 7-111: Aorta of PO A fraction treated animals after oil red 0 stdningshowlng areas of fat deposltlon
- 8 Summary and conclusion
- References