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Page: 197
 
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  • TITLE
  • CERTIFICATE
  • DECLARATION
  • ACKNOWLEDGEMENT
  • CONTENTS
  • INTRODUCTION AND REVIEW
  • Cataracts
  • 1. Developrental cataracts
  • 2. Degenerative cataracts
  • senile cataract
  • 1. Incipient stage
  • 2. Intumescent stage
  • 3. Mature stage
  • 4. Hypermature stage
  • MATERIALS AND METHODS
  • Stages of cataract
  • (a) Immature cataract
  • (b) Mature cataract
  • (c) Hypermature cataract
  • Collection and transportation of samples
  • (a) Blood samples
  • (b) Lens samples
  • Preparation of freezing mixture
  • Preparation of haerolysate
  • Preparation of lens homogenate
  • Preparation of Drabkins solution
  • Preparation of soluble and insoluble protein fractions of lens
  • Determination of transketolase activity
  • Preparation of solutions
  • incubation
  • anthrone method
  • Orcinol lethod
  • Determination of ribose content
  • Assay of enzyme by macro method
  • Assay of glutathione reductase
  • Glutathione peroxidase
  • RESULTS
  • Blood sugar
  • Lens Protein
  • Glucose 6-phosphate dehydrogenase
  • Transketolase
  • Transaldolase
  • Glutathione reductase
  • Reduced glutathione
  • Correlation analysis
  • DISCUSSION
  • Incidence of Cataract
  • Blood Sugar
  • Lens Protein
  • Glucose 6-phosphate dehydrogenase
  • Transketolase
  • Transaldolase
  • Glutathione Reductase
  • Reduced Glutathione
  • Glutathione Peroxidase
  • SUMMARY AND CONCLUSION
  • BIBLIOGRAPHY