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Title
DEDICATION
CERTIFICATE
DECLARATION
ACKNOWLEDGEMENT
LIST OF TABLES
LIST OF FIGURES
LIST OF PLATES
CONTENTS
1. Introduction
Bufo melanostictus
Diagnosis
Review of earlier work done
Justification of the present work
2. Status, Habitat & Distribution
Study area
Topography and Climate
1. Meterological data of the study area
Distribution
Habitat
1. Map of Pathanamthitta District
Sexual dimorphism
Size
Secondary Sexual characters
Sex ratio
Present status of B. melanostictus
3. Food and feeding habits
Introduction
Matrials and Methods
Results
2. Stomach contents of B.melanostictus expressed as percentage of total body weight with respect to sex and month
3. Food spectrum of Bufo melanostictus
4. Seasonal variation in the food item of B. melanostictus
5. Percentage of food items recovered from the stomach of B. melanostictus during Jan 1991 to Dec. 1992
6. Monthly variation in the feeding activity of B. melanostictus
2. Annual consumption of food items by B. melanostictus
7. Correlation metrics of feeding activity of B. melanostictus
8. Economically important organisms recovered from the gut of B. melanostictus
DISCUSSION
4. Male reproductive cycle
Introduction
Materials and Methods
Results
Gonadosomatic index
Relative testicular weight
9. Seasonal variation in the GSI, relative testicular weight, testis diameter, Testis tubule diameter, Ley dig cell nuclear diameter and fat body weight of B. melanostictus
3. Seasonal changes in the GSI, diameter of the testis, seminiferous tubule, Leydig cell nuclear diameter and fat body weight of B. melanostictus in relation to temperature, humidity and rainfall
Testis diameter
Seminiferous tubule diameter
Leydig cell nuclear diameter
Fat body cycle
10. Correlation metrics between testicular activity of B. melanostictus
Spermatogenetic stages
11. Correlation metrics between testis diameter, tubule diameter and Leydig cell nuclear diameter of B. melanostictus
12. Seasonal variation in the spermatogenetic stages of B. melanostictus
1. Photomicrographs of sections of testis of B. melanostictus
4. Seasonal variation in spermatogenetic stages of B. melanostictus
Dicussion
5. Female reproductive cycle
Introduction
Materials and methods
Results
Body weight
Gonadosomatic Index
Ovarian cycle
2. Photomicrographs of sections of ovary of B. melanostictus
13. Seasonal variation in the ovarian activity of B. melanostictus
5. Seasonal variation in the fat body weight, ovary weight, oviduct weight and GSI of B.melanostictus
6. Seasonal variation in the diameter of largest oocyte of B. melanostictus
1. Pre-breeding or preparatory phase
2. Breeding phase
3. Post breeding phase
Oviduct cycle
Fat body cycle
14. Correlation co-efficient between body weight, ovary mass and various factors of ovarian activity of B. melanostictus
DISCUSSION
6. Effects of some pesticides on the hatching rate, survival and growth of tadpoles
Introduction
Materials and methods
15. List of pesticides used in the present study
Results
16. Effect of endosulfan on the hatching rate of B. melanostictus
17. Effect of hinosan on the hatching rate of B. melanostictus
7. Effect of endosulfan on the mortality of tadpoles of B. melanostictus
8. Effect of hinosan on the mortality of tadpoles of B. melanostictus
18. 96 hr LC50, 95% confidence limits, safe concentration, slope value and regression equation for tadpoles of Bs melanostictus exposed to pesticides
9. Effect of endosulfan on larval survival of B. Melanostictus
10. Effect of hinosan on larval survival B. Melanostictus
19. Effect of endosulfan on the weight and length of tadpoles of B. melanostictus
20. Effect of hinosan on the weight and length of tadpoles of B. melanostictus
11. Effect of endosulfan on the percentage of length decrease of tadpoles from their control values
12. Effect of endosulfan on the percentage of weight decrease of tadpoles from their control values
13. Effect of hinosan on the percentage of length decrease of tadpoles from their control values
14. Effect of hinosan on the percentage of weight decrease of tadpoles from their control values
21. Effect of pesticides on the percentage of length and decrease of tadpoles from their control values
22. Effect of pesticides on the percentage of weight decrease of tadpoles from their control values
DISCUSSION
7. Histopathological changes in the gonad, liver and kidney of adults exposed to sublethal concentrations of some pesticides
Introduction
Materials and methods
Results
15. Effect of endosulfan on the mortality of adult B. melanostictus
16. Effect of hinosan on the mortality of adult B. melanostictus
Testis
23. 24 hr LD50, fiducial limits, slope value and regression equation for B.melanostictus adults exposed to pesticides
24. Summerised account of histopathological effects produced by sublethal concentrations of endosulfan and hinosan in different organs of B. melanostictus
Exposure to 0.24 ppm/gm body weight of endosulfan
Exposure to 0.48 ppm/gm body weight of endosulfan
3. Photomicrographs of sections of testis of B.melanostictus exposed to endosulfan (x 250)
Exposure to 0.054 ppm/gm body weight of hinosan
Exposure to 0.108 ppm/gm body weight of hinosan
4. Photomicrographs of sections of testis of B. melanostictus exposed to hinosan (x 250)
25. Effect of endosulfan on spermatogenetic stages of B. melanostictus
26. Effect of hinosan on spermatogenetic stages of B.melanostictus
17. Effect of endosulfan on the spermatogenetic stages (0 and I) of B. melanostictus
18. Effect of endosulfan on the spermatogenetic stages (II and III) of B.melanostictus
19. Effect of endosulfan on the spermatogenetic stages (IV and V) of B. melanostictus
20. Effect of hinosan on the spermatogenetic stages (0 and I) of B. melanostictus
21. Effect of hinosan on the spermatogenetic stages (II and III) of B. melanostictus
22. Effect of hinosan on the spermatogenetic stages (IV and V) of B.melanostictus
Ovary
Exposure to pesticides
5. Photomicrographs of sections of ovary of B.melanostictus exposed to pesticides (x 100)
Gonadosomatic Index
27. Effect of endosulfan on the GSI of male B.melanostictus
23. Effect of endosulfan on the GSI of male B. melanostictus
24. Effect of endosulfan on the GSI of female B. melanostictus
28. Effect of endosulfan on the GSI of female B. melanostictus
29. Effect of hinosan on the GSI of male B.melanostictus
25. Effect of hinosan on the GSI of male B. melanostictus
26. Effect of hinosan on the GSl of female B. melanostictus
Liver
30. Effect of hinosan on the GSI of female B. melanostictus
Exposure to 0.24 ppm/gm body weight of endosulfan
Exposure to 0.48 ppm/gm body weight of endosulfan
6. Photomicrographs of sections of liver of B.melanostictus exposed to endosulfan (x 250)
Exposure to 0.054 ppm/gm body weight of hinosan
Exposure to 0.108 ppm/gm body weight of hinosan
7. Photomicrographs of sections of liver of B. melanostictus exposed to hinosan (x 250)
Hepatosomatic Index
31. Effect of endosulfan on the HSI of B.melanostictus
32. Effect of hinosan on the HSI of B. melanostictus
27. Effect of endosulfan on the HSI of BR melanostictus
28. Effect of hinosan on the HSI of B. melanostictus
Kidney
Exposure to 0.24 ppm/gm body weight of endosulfan
Exposure to 0.48 ppm/gm body weight of endosulfan
8. Photomicrographs of sections of kidney of B. melanostictus exposed to endosulfan (x 250)
Exposed to 0.054 ppm/gm body weight of hinosan
Exposed to 0.108 ppm/gm body weight of hinosan
9. Photomicrographs of sections of kidney of Bs melanostictus exposed to hinosan (x 250)
Dicussion
8. Summary and Conclusions
SUMMARY
CONCLUSlON
9. References