Effect of glutathione enriched herbal formulation on experimental diabetic model: possible role in age associated pathogenesis

Accession Number	T 4860
Call Number
Research Scholar	Sheethal S
Guide	Ratheesh M
Year	2023
Centre of Research	Department of Biochemstry, St Thomas College, Pala
Scholar Address	Pankajamandiram, Kuttikkattukara P.O, Udyogamandal- 683 501
Guide Address	Assistant Professor, Department of Chemistry, St. Thomas College, Palai
Branch of Study	Biochemistrty
Keywords	glutathione enriched herbal formulation experimental diabetic model pathogenesis Diabetes mellitus History of diabetes mellitus Types of diabetes mellitus Type 1 diabetes Type 2 diabetes Gestational diabetes Epidemiology of diabetes Classical signs and symptoms of diabetes Fasting plasma glucose Oral glucose tolerance test Glycated hemoglobin Prevention and treatment of diabetes Pharmacological therapy Biguanides Sulfonylurease Alpha-glucosidase inhibitors Thiazolidinediones Glucose metabolism Organs in glucose homeostasis Glucose transporters Facilitated glucose transporters Sodium glucose cotransporters Insulin biosynthesis Insulin secretion Insulin mechanism of action Pathogenesis of type 2 diabetes Glucotoxicity Lipotoxicity Free radicals Types of free radicals Biological activities of free radical Formation of free radicals NADPH Oxidase Electron transport chain Mitochondria NO production Oxidative stress Oxidative stress in diabetes Molecular and signalling pathways in diabetes Glycolysis Advanced glycation endproduct pathway Interaction of AGE with its receptor Protein Kinase C pathway Chemical induction of diabetes in animal models Antioxidants Level of antioxidant action Types of antioxidants Water soluble antioxidants Fat soluble antioxidants Endogenous antioxidants Exogenous antioxidants Superoxide dismutase Catalase Glutathione peroxidase Glutathione Ascorbic acid Tocopherol Alternative therapies for diabetes Antioxidant therapy Herbalism Curcuma longa Capsicum frutescens Murraya koenigii Vitis vinifera Antibodies Preparation of glutathione enriched polyherbal formulations Oral glucose tolerance test Formulation of GEF tablet Carr’s Compressibility Index Hausner’s Ratio Preparation of polyherbal tablets Microbiological Load Analysis Characterization of GEF tablets UV-Visible spectroscopy FT-IR analysis LC-MS/MS analysis Sample preparation In-vivo induction of diabetes Insulin resistance in L6 cell line Analytical procedure Assay of Catalase Assay of superoxide dismutase Assay of Glutathione peroxidase Estimation of glutathione content Estimation of nitrite level α- amylase inhibitory activity α- glucosidase inhibitory activity Cell cytotoxicity assay Intracellular ROS generation Lipid Peroxidation Glucose uptake assay Estimation of HbA1c level Enzyme linked immuno-sorbent assay Western blot analysis RT-PCR analysis Total RNA isolation cDNA synthesis Thermo cycling procedure Agarose gel electrophoresis Statistical Analysis Preparation and Optimization of Glutathione Enriched Herbal Formulation Experimental design for OGTT Toxicological assessment of Glutathione Enriched Polyherbal Formulation Histopathology of internal organs Characterisation and Formulation of Glutathione Enriched Polyherbal Formulation Plant materials Formulation Studies Microbial load analysis in GEF UV-Vis spectrum analysis LC-MS chromatogram analysis Anti-diabetic effect of glutathione enriched polyherbal formulation Streptozotocin induced experimental model Inhibiting AGE/NF-kB pathway Estimation of Glycated haemoglobin Enzyme-linked immunosorbent assay Western blot analysis of Glo-I mRNA expression of NF-κB and iNOS Histopathological analysis of liver tissue Histopathological analysis of pancreas tissue Effect of glutathione enriched polyherbal formulation Streptozotocin induced diabetic model Regulating oxidative stress PKC pathway Liver enzyme markers Antioxidant activity TBARS level Nitrite level GLUT-2 gene expression in liver Insulin sensitizing effect High glucose-high insulin induced L6 skeletal muscle cells Enzymatic Activity Cell culture Cytotoxicity analysis Induction of insulin resistance in L6 cells Glucose uptake assay Protein expression of IRS-1 Akt GLUT-4 Cell viability analysis Validation of insulin resistance model Morphological observation of L6 myoblast cells Intracellular ROS level TBARS level