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Full Screen- TITLE
- DEDICATION
- CERTIFICATE
- CERTIFICATE
- DECLARATION
- A word of gratitude..
- CONTENTS
- I. Introduction
- Leptospirosis
- Epidemiology
- Plate I Transmission and maintenance of leptospira in the environment
- Pathogenesis
- Clinical manifestations
- Mortality
- Diagnosis
- Treatment
- The Organism
- Plate 2: Dark-filled illumination
- Plate 3: Diagrammatic representation of the coils
- Classification
- Past work
- Plate 4: MAT reactions
- Genomic identification methods
- Polymerase Chain Reaction
- Study area
- Fig 1 Map of the study area
- II. Review of Literature
- III. Aim of the Study
- IV. Materials and Methods
- Reference strains
- Culture conditions and DNA extraction
- DNA Extraction
- General procedure followed in Electrophoresis:
- Multiplex PCR
- AP-PCR
- V. Results
- Plate No.5: DNA extract electrophoresis
- Plate No.6: MP-PCR
- Plate No.7: MP - PCR
- Plate No. 8: MP - PCR
- Plate No.9 MP - PCR
- Plate No. 10: MP -PCR
- Plate No.11: AP-PCR (Reference strains)
- Plate No.12 AP-PCR (Reference strains)
- Plate No.13: AP- PCR
- Plate No.14 AP-PCR Reference serovars and Isolates
- Plate No.15: AP-PCR
- Plate No.16: AP-PCR
- Plate No.17: AP-PCR
- Plate No.18: AP-PCR
- Plate No.19: AP-PCR
- Plate No.20: AP-PCR
- Plate No.21: AP-PCR
- Plate No.22: AP-PCR
- Chart 1: Serovars Identified
- Table 1 Genospecies distribution of serovars
- Chart 2: Genospecies prevalence in the study
- Chart 3: Clinical Complications
- VI. Discussion
- Plates
- Locally identified autumnalis isolates used for reference
- References
- APPENDIX
- Published Papers
- Important abbreviations
- Thanks