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  • TITLE
  • DEDICATION
  • DECLARATION
  • CERTIFICATE
  • ACKNOWLEDGEMENT
  • CONTENTS
  • 1. INTRODUCTION
  • 2. MATERIALS AND METHODS
  • I. GERMINATION STUDIES
  • a. Germination Percentage
  • b. Radicle and Hypocotyl length
  • II. COTYLEDONARY STUDIES
  • a. Stomatal index and stomata1 freauency
  • b. Chlorophyll and carotenoid content (Cotyledonary) (Arnon 1949)
  • III. MORPHOLOGICAL STUDIES
  • IV. EPIDERMAL STUDIES - MATURE LEAVES
  • a. Stomatal index and stomatal freauency
  • V. PHYTOMASS AND PRODUCTIVITY
  • V I. YIELD STUDY
  • V I I. SEM STUDIES - SEEDS
  • V I I I. HISTOCHEMICAL STUDIES - MATURE LEAVES
  • a. Qualitative analysis
  • b. Quantitative analysis
  • 3. OBSERVATIONS
  • I. GERMINATION STUDIES
  • a. Germination percentage
  • b. Radicle and Hypocotyl length
  • II. COTYLEDONARY STUDIES
  • a. Stomatal index and stomatal frequency
  • Table 1. Physico-chemical characteristics of pulp and paper mill effluent.
  • Table: 2. Seed germination percentage of the plants treated with different dilutions of the effluent.
  • Table: 3 Radicle and Hypocotyl length of Abelnoschus esculentus, (L.) Noench treated with different dilutions of the effluent.
  • Table 4 Radicle and HypocotyI length of Cucusis sativus, L. treated with different dilution of the effluentt.
  • Table 5 Radicle and Hypocotyl length of Lycopersicum esculentum, Mill.treated dilutions of the effluent
  • Table 6 Radicle and Hypocotyl length of Sesamus indicus L.. treatrd with different dilutions of the efflumt.
  • Table: 7 Stonatal index and stomatal frequency (cotyledon) of plants treated with different dilutions of effluent.
  • Table: 8 Cotyledonary chlorophyll and carotenoid contents of Abelmoschus esculentus, (L.) Moench treated with different dilutions of the effluent.
  • Table: 9 Cotyledonary chlorophyll and carotenoid contents of Cucumis sativus. L.. treated with different dilutions of the effluent.
  • Table: 10 Cotyledonary chlorophyll and carotenoid contents of Lycopersicum esculentum, M i l l. treated with different dilutions of the effluent
  • Table: 11 Cotyledonary chlorophyll and carotenoid contents of Sesamum indicum, L. treated with different dilutions of the effluent.
  • III. MORPHOLOGICAL STUDIES
  • Fig.1ABELMOSCHUS ESCULENTUS, (L.) Moench - Stem length (cm)
  • Fig.2ABELMOSCHUS ESCULENTUS, (L.) Moench - Root length (cm)
  • Fig.3ABELMOSCHUS ESCULENTUS, (L.) Moench - Number of Leaves
  • Fig.4CUCUMIS SATIVUS, L. - Stem length (cm)
  • Fig.5CUCUMlS SATIVUS, (L.) - Root length (cm)
  • Fig.6CUCUMIS SATIVUS, L. - Number of Leaves
  • Fig.7LYCOPERSICUM ESCULENTUM, Mill. - Stem length (cm)
  • Fig.8LYCOPERSICUM ESLULENTUM. Mill. - Root length (crn)
  • Fig.9LYCOPERSICUM ESCULE.NTUM, Mill - Number of Leaves
  • Fig.10SESAMUM INDICUM, (L.) -Stem length (cm)
  • Fig.11SESAMUM INDICUM, L. - Root length (cm)
  • Fig.12SESAMUM INDICUM, L. - Number of Leaves
  • IV. EPIDERMAL STUDIES - MATURE LEAVES
  • a. Stomatal index and stomatal frequency
  • Table: 21 Stwatal index and stomatal frequency (oature leaves) of the plants treated with different dilutions of effluent.
  • V. PHYTOMASS AND PRODUCTIVITY
  • Fig.13ABELMOSCHUS ESCULENTUS, (L.) Moench - Phytomass - Stem (gm)
  • Fig.14ABELMOSCHUS ESCULENTUS, (L.) Moench - Phytomass - Root (gm)
  • Fig.15ABELMOSCHUS ESCULENTUS, (L.) Moench - Phytomass - Leaf (gm)
  • Fig.16ABELMOSCHUS ESCULENTUS. (L) Moench - Total phytornass
  • Fig.17ABELMOSCHUS ESCULENTUS, &.) Moench NPP (gm)
  • Fig.18CUCUMlS SATIVUS, L. - Phytornass - Stem (grn)
  • Fig.19CUCUMIS SATIVUS, L. - Phytomass - Root (grn)
  • Fig.20 CUCUMIS SATIVUS, L. - Phytomass - Leaf (gm)
  • Fig.21CUCUMlS SATIVUS. L. - Total phytomass
  • Fig.22CUCUMlS SATIVUS, L. - NPP (gm)
  • Fig.23LYCOPERSICUM ESCULENTUM, Mill. - Phytornass - Stem (grn)
  • Fig.24LYCOPERSlCUM ESCULENTUM Mill. - Phytomass - Root (grn)
  • Fig.25LYCOPERSICUM ESCULENTUM, (L.) Moench - Phytornass - Leaf (gm)
  • Fig.26LYCOPERSICUM ESCULENTUM, Mill. - Total phytornass (gm)
  • Fig.27LYCOPERSICUM ESCULENTUM, Mill.
  • Fig.28SESAMUM INDICUM. L. - Phytomass - Stem (gm)
  • Fig.29SESAMUM INDICUM, L. - Phytornass - Root (grn)
  • Fig.30SESAMUM INDICUM, L. - Phytornass - Leaf (gm)
  • Fig.31SESAMUM INDICUM, L. - Total phytornass (grn)
  • Fig.32SESAMUM INDICUM.L - NPP (gm)
  • VI. YIELD STUDIES
  • a. Number of flowers
  • Fig.33ABELMOSCHUS ESCULENTUS. (L.) Moench - Number of flowers
  • Fig.34ABELMOSCHUS ESCULENTUS, (1.M) oench - Number of fruits
  • Fig.35ABELMOSCHUS ESCULENTUS, (L) Moench - Dry weight of fruits
  • Fig.36CUCUMlS SATIVUS, L. - Number of flowers
  • Fig.37CUCUMlS SATIVUS, L. - Number of fruits
  • Fig.38CUCUMIS SATIVUS, L. - Dry weight of fruits
  • Fig.39LYCOPERSlCUM ESCULENTUM, Mill. - Number of flowers
  • Fig.40LYCOPERSlCUM ESCULENTUM, Mill. - Number of fruits
  • Fig.41LYCOPERSICUM ESCULENTUM, Mill. - Dry weight of fruits
  • Fig.42SESAMUM INDICUM, L. - Number of flowers
  • Fig.43SESAMUM INDICUM, L. - Number of fruits
  • Fig.44SESAMUM INDICUM, L. - Dry weight of fruits
  • VII. SEM STUDIES - SEEDS
  • PLATE -1 SCANNING ELECTRON MICROGRAPHS OF SEEDS OFABELMOSCHUS ESCULENTUS, (L.) Moench
  • PLATE - 2 SCANNING ELECTRON MICROGRAPHS OF THE SEEDS OFCUCUMIS SATIVUS, L.
  • PLATE - 3 SCANNING ELECTRON MICROGRAPHS OF THE SEEDS A-C: LYCOPERSICUM ESCULENTUN, Mill. D-F: SESAMUM INDICUM, (L.)
  • VIII. HISTOCHEMICAL STUDIES - MATURE LEAVES
  • a. Qualitative analysis
  • PLATE - 4 HISTOCHEMICAL LOCALISATION OF INSOLUBLE POLYSACCHARIDES -IN FOLIAR EPIDERM; (PAS REACTION)
  • PLATE - 5 HISTOCHEMICAL LOCALISATION OF INSOLUBLE POLYSACCHARIDES IN FOLIAR EPIDERMIS (PAS REACTION)
  • PLATE - 6 HISTOCHEMICAL LOCALISATION OF SULPHATED AND CARBOXYLATED POLYSACCHARIDES IN FOLIAR EPIDERMIS
  • PLATE - 7 HISTOCHEMICAL LOCALISATION OF SULPHATED AND CARBOXYLATED POLYSACCHARIDES IN FOLIAR EPIDERMIS
  • PLATE - 8 HISTOCHEMICAL LOCALISATION OF STARCH IN FOLIAR EPIDERMIS (I2KI REACTION)
  • PLATE - 9 HISTOCHEMICAL LOCALISATION OF STARCH IN FOLIAR EPIDERMIS (I2KI REACTION)
  • PLATE - 10 HISTOCHEMICAL LOCALISATION OF PROTEIN IN FOLIAR EPIDERMIS MERCURIC BROMOPHENOL BLUE REACTION
  • PLATE - 11 HISTOCHEMICAL LOCALISATION OF TOTAL PROTEINS IN FOLIAR EPIDERMIS (MERCURIC BROMOPHENOL BLUE REACTION)
  • b. Quantitative analysis
  • 1.Chlorophyll and carotenoid contents
  • 2- Carbohydrate content
  • 3. Protein content
  • Fig.45 ABELMOSCHUS ESCULENTUS, (L.) Moench (Mature leaves) - Chlorophyll-a content
  • Fig.46 ABELMOSCHUS ESCULENTUS. (L.) Moench (Mature leaves) - Chlorophyll-b content
  • Fig.47 ABELMOSCHUS ESCULENTUS, (L.) Moench (Mature leaves) - Total Chlorophyll content
  • Fig.48 ABELMOSCHUS ESCULEINTUS, (L.) Moench (Mature leaves) - Carotenoid content
  • Fig.49 CUCUMIS SATIVUS, L. (Mature leaves) - Chlorophyll-a content
  • Fig.50 CUCUMIS SATIVUS, L. (Mature leaves) - Chlorophyll-b content
  • Fig.51 CUCUMlS SATIVUS, L. (Mature leaves) - Total Chlorophyll content
  • Fig.52 CUCUMlS SATIVUS, L. (Mature leaves) - Carotenoid content
  • Fig.53 LYCOPERSICUM ESCULENTUM, Mill. (Mature leaves) - Chlorophyll-a content
  • Fig.54 LYCOPERSICUM ESCULENTUM, Mill. (Mature leaves) - Chlorophyll-b content
  • Fig.55 LYCOPERSICUM ESCULENTUM, Mill (Mature leaves) - Total chlorophyll
  • Fig.56 LYCOPERSICUM ESCULENTUM, Mill (Mature leaves) - Carotenoid content
  • Fig.57 SESAMUM INDICUM, L. (Mature leaves) - Chlorophyll-a content
  • Fig.58 SESAMUM INDICUM, L. (Mature leaves) - Chlorophyll-b content
  • Fig.59 SESAMUM INDICUM, L. (Mature leaves) - Total Chlorophyll content
  • Fig.60 SESAMUM INDICUM, L. (Mature leaves) - Carotenoid content
  • Fig.61 ABELMOSCHUS ESCULENTUS, (L.) Moench - Carbohydrate content
  • Fig.62 CUCUMlS SATIVUS, L. - Carbohydrate content
  • Fig.63 LYCOPERSICUM ESCULENTUM, Mill. - Carbohydrate content
  • Fig.64 SESAMUM INDICUM, L. - Carbohydrate content
  • Fig.65 ABELMOSCHUS ESCULENTUS, (L.) Moench - Protein content
  • Fig.66 CUCUMlS SATIVUS, L. - Protein content
  • Fig.67 LYCOPERSICUM ESCULENTUM, -Mill. - Protein content
  • Fig.68 SESAMUM INDICUM, L. - Protein content.
  • 4. DISCUSSION
  • 5. SUMMARY AND CONCLUSION
  • 6. LITERATURE CITED