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Full Screen- TITLE
- CERTIFICATE
- DECLARATION
- ACKNOWLEDGEMENT
- DEDICATION
- CONTENTS
- ABBREVIATIONS
- INTRODUCTION
- Utilization of reeds
- Traditional Sector
- Industrial sector
- Factors causing reed forest degradation
- a) Ochlandra travancorica
- Propagation
- Anatomy
- (b) Ochlandra travancorica var hirsuta
- Aims and objectives of present research endeavour
- REVIEW OF LITERATURE
- Bamboo tissue culture a Review
- Micropropagation
- Adventitive Shoot Formation
- Somatic embryogenesis
- Artificial seeds
- In vitro Flowering
- Factors affecting success of in vitro propagation
- 1. Explant
- b. Surface sterilization
- Culture Media
- Growth Regulators
- Vitamins
- Other Organic Compounds
- Culture enviornment &.conditions
- Genotype
- Root Induction
- Acclimatisation and Planting out
- MATERIALS AND METHODS
- Plant Material
- Chemicals and Glasswares
- Media preparation and sterilization
- Inoculation and subculture
- Histological studies
- Growth measurement and statistical analysis
- Ochlandra travancorica
- I Micropropagation
- a Collection and Preparation of Explants
- b. Sterilization techniques of Explants
- c. Culture Media
- d Inoculation of Explants
- e Axillary bud proliferation and multiple shoot induction
- f. Elongation of in vitro shoots
- g Rooting
- Hardening and plantouts
- IV Callus culture
- a Explants per callus induction
- b. Media for callus induction
- Callus isolation and subculture
- Organogenesis from the callus
- III In vitro flower induction
- Media for flower induction
- IV Histological studies
- Ochlandra travancorica var hirsuta
- I Micro propagation
- a Explant selection and surface sterilization
- Surface sterilisation
- b. Culture media
- Axillary bud proliferation and multiple shoot production
- Experiment No. I The effect of IAA on multiple shoot induction
- Experiment No.II The effect of cytokinin on multiple shoot induction
- Ixperiment No. III
- Rooting of shoots
- Hardening and plantouts
- IV Callus culture
- Explant selection
- Culture media
- Experiment No. I
- Experiment II
- III HIistological studies
- RESULTS
- Ochlandra travancorica
- I Micropropagation
- Size of the Explants
- Standardization of Media and Plant growth regulators
- The effect of BAP on multiple shoot induction
- Effect of Kinetin in Multiple shoot production
- Effect of IAA on multiple shoot induction
- The effect of coconut milk
- Effect of BAP with other auxins
- Shoot elongation
- Rooting
- Hardening and plantouts
- II Callus culture
- Effect of phytohormones in callus induction
- Effect of 2-4 D in callus induction
- Effect of 2-4D with BAP
- Effect of 2-4 D with kinetin
- Effect of 2-4 D along with 2 ip
- Effect of other plant growth regulators
- Organogenesis from callus
- In vitro flower induction
- HISTOLOGY
- a) Shoot
- b) Callus
- Ochlandra travancorica var hirsuta
- Micro propagation
- a) Size of the explants
- Multiple shoot induction
- 1. Experiment No.1 The effect of explants with Auxin 1AA
- 2. Experiment No.11
- The effect of BAP with Auxins
- 3. Experiment No.111
- In vitro shoot elongation
- Rooting
- Hardening
- Callus Culture
- Experiment No.1
- Experiment No.11
- Histology
- Shoot
- Callus
- Table I Chemical composition of various culture media used for rrt vrtro propagation ofOchlnr~dratr ai~crricorrcn
- Table III Effect of explant size in axillary bud proliferation in 0. lrni~atlcorrcn
- Table III The effect of size and percentage of explant establishment
- Table IV Effect of BAP concentrations on shoot multiplication in O.travancorica
- Table V Effect of different auxins on multiple shoot production in O.travancorica (BAP 4 mg/l)
- Table VI Effect of different auxins on iri vilro rooting in 0.travancorica
- Table VII Effect of different concentration of 2-4 D on growth and fresh weight (mg) of callus on leaf, leaf sheath and internodal explants of O.travancorica
- Table VIII Effect of auxin/cytokinins on callus regeneration in O.travancorica
- Table IX Media composition for in vitro flower induction in O.travancorica
- Table X The effect of auxin IAA on axillary bud proliferation and multiple shoot induction in O.travancorica var hirusta
- Table XI The effect of different cytokinins on axillary bud proliferation and multiple shoot induction in O.travancorica var hirusta
- Table XII Effect of different auxins on in vitro rooting of O.travancorica var hirusta
- Table XIII Callus formation in different explants with varying hormones in O.travncorica var hirusta
- Fig. I Ochlandra travancorica
- Fig. II Ochlandra travancorica
- Fig. III Ochlandra travancorica
- Fig. IV Ochlandra travancorica
- Fig. V Ochlandra travancorica
- Fig. VI Ochlandra fravancorica
- Fig. VII Ochlandra travancorica
- Fig. VIII Ochlandra travancorica
- Fig. IX Ochlandra travancorica (X 400)
- Fig. X Ochlandra travancorica
- Fig. XI Ochlandra travancorica
- Fig. XII Ochlandra travancorica
- Fig. XIII Ochlandra travancorica
- Fig. XIV Ochlandra travancorica var hirsuta
- Fig. XV Ochlandra travancorica var hirsuta
- Fig. XVI Ochlandra travancorica var hirsuta
- Figire XVII Ochlandra travancorica var hirsuta
- Fig. XVIII Ochlandra travancorica var hirsuta
- Fig. XIX Ochlandra travancorica var hirsuta
- Fig. XX Ochlandra travancorica var hirsuta
- Fig. XXI Ochlandra travancorica var hirsuta
- Fig. XXII Ochlandra travancorica var hirsuta
- DISCUSSION
- Ochlandra travancorica
- Micrpropagation
- 1. Culture Establishment
- 2. Multiplication
- 3. Rooting
- 4. Hardening and plant outs.
- The observations recorded in the present work.
- Callus Culture
- In vitro Flowering
- Ochlandra travancorica var hirsuta
- Micropropagation
- Callus culture
- SUMMARY
- Ochlandra travancorica
- Oclrlandra travancorica var hirsuta
- REFERENCES