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  • Title
  • Dedication
  • Declaration
  • Certificate
  • Certificate (Director)
  • Acknowledgement
  • Abstract
  • Preface
  • Contents
  • List of Tables
  • List of Figures
  • Abbreviations
  • 1. General introduction
  • 1.1.1. Biosurfactants
  • 1.1.2. Rhamnolipid biosurfactants
  • 1.1.3. Pulp and paper industry
  • 1.2.1. Sources and types of biosurfactants
  • 1.2.2. Low cost substrates or cost-effective carbon sources
  • 1.2.3. Applications of rhamnolipids
  • 1.2.4. Surfactants in pulp and paper industry
  • 2. Materials and Methods
  • 2.1. Screening and selection of cost-effective natural substrates for biosurfactant production under submerged fermentation
  • 2.2. Screening and isolation of biosurfactant producing bacterial strains
  • 2.3. Selection of the most efficient biosurfactant producing strain on selected cost-effective carbon sources
  • 2.4. Identification of the selected bacterial strains
  • 2.5. Qualitative and quantitative analytical methods for rhamnolipid production
  • 2.6. Optimization of the process parameters for the biosurfactant production
  • 2.7. Growth curve of the bacterial strains
  • 2.8. Rhamnolipid recovery
  • 2.9. Rhamnolipid purification
  • 2.10. Characterization of rhamnolipid by fast-atom bombardment mass spectroscopy (fabms)
  • 2.11. Application of the crude rhamnolipid in the deinking of old newspaper
  • 3. Results
  • 4. Discussion
  • 5. Summary, Conclusion and Future prospects
  • 6. Bibliography
  • 7. Appendices