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Thesis Details
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TITLE
CERTIFICATE
DECLARATION
ACKNOWLEDGEMENT
DEDICATION
CONTENTS
1 INTRODUCTION
2 REVIEW OF LITERATURE
2.1. Effect of location on VAM distribution
2.2. Effect of season on VAM colonisation and sporepepalation
2.3. Comparison of forest and rubber growing soils
2.4. Effect of soil factors on the distribution of VAM fungi
2.4.1. Soil temperature
2.4.2. Soil moisture
2.4.3. Soil organic matter
2.4.4. Soil pH
2.5. Effect of cultural operations on VAM colonisationand spore population
2.5.1. Fertilizer application
2.5.2. Fungicide application
2.5.3. Pesticide application
2.5.4. Weedicide application
2.6. Soil solarisation
2.7. Dissemination of VAM spores
2.7.1. Active dissemination
2.7.2. Passive dissemination
2.7.2.1. Biotic agents
2.7.2.2. Abiotic agents
3 MATERIALS AND METHODS
3.1. Collection of soil and root samples
3.1.1. Enumeration of VAM spore population
3.1.2. Identifxation of spores
3.1.3. Assessment of root colonisation (Phillips and Hayman, 1970)
3.1.4. Seasonal variability on VAM spore count in rubbergrowbg soils and colonisation in Hevea roots
3.2. Determination of organic carbon in soil
3.3. Determination of soil pH
3.4. VAM spore population and root colonisation in Heveaplantations under different period of cultivation
3.5. VAM spore population and root colonisation inrubber soils under 3 different cycles of cultivation
3.6. VAM spore population at different soil depths
3.7. Comparison of forest soil and rubber plantation soilfor VAM spore distribution
3.8. Effect of soil factors on VAM spore count and rootcolonisation
3.8.1. Effect of temperature
3.8.2. Effect of soil moisture on VAM spore count and rootcolonisation
3.8.3. Effect of different sources of organic matter in soil onVAM spore count and root colonisation
3.9. Effect of cultural operations on VAM spore countand root colonisation
3.9.1. Effect of fertilizers
3.9.2. Effect of plant protection chemicals
3.9.2.1. Fungicides
3.9.2.2. Insecticides
3.9.2.3. Weedicides
3.9.3. Effect of soil solarisation on VAM
3.10. Studies on the dissemination of.VAM fungi
3.10.1. Biotic agents
3.10.2. Abiotic agents
Plate 1. Earthworm castings in soil
Plate 2. Termitary on a rubber tree
Plate 3. Termitary in soil
Plate 4. Ant mound.
Plate 5. Wasp nest
Plate 6. Rainwater flowing through a rubber field.
Plate 7. spore trap (Insight - side view from right)
Plate 8. Spore trap erected in a rubber plantation.
4 EXPERIMENTALRESULTS
4.1. Influence of location and season on VAM sporepopulation in rubber growing soils of Kerala
Fig. 1. Sites of soil sample collection.
4.2. Effect of location and season on Hevea rootcolonisation by VAM
Fig.2 Effect of season on the occurence of different generaof VAM spores in H e w plantations
4.3. Effect of soil pH and organic carbon on VAM sporecount in soil and root colonisation in Hevea
Plates 9-12 Spores of different species of Glomus sp (x 160)
Plate 13. Spore of Acmlospora sp. (x 160)
Plate 14. Spore of Sclerocystis sp. (x 160)
Plate 15. Spore of Gigaspora sp. (xl60)
Plate 16. Enlarged view of Giga.~porasp.spore (x340) showing bulbousSubtending hypha
Plate 17. Fungal hyphae showing: appresorium (a) on Hevea root (x 160)
Plate 18. Root colonisation in Ht~vea showing vesicles (x 160)
Plate 19. Root colonisation in sorghum showing arbuscules (x 160)
4.4. VAM spore ppttktion and root colonisation inHevea plantrations under different period ofcultivation
4.5. Spore m a t i o n and root colonisation in the soilsof three BiPferent cycles of Hevea cultivation
Fig 3. VAM colonisation ofHevea roots under differentyears of cultivation
Fig 4. VAM spore count in rubber plantation soil underdifferent years of cultivation
Fig. 5. Per cent occurrence of different genera of VAMspores in Hmea plan tations under different years ofcultivation
Fig.6. VAM Spare count in soil of ru bber plantationunder different cultivation cycle
Fig 7. VAM colonisation in H m mots under differentcultivation cycle.
4.6. Influence of soil depth on VAM spore population
4.7. Comparative studies on VAM spore count in forestand rubber growing soils
Fig.8 Influence of soil depth on VAM spore count inrubber plantation
4.8. Effect of soil factors on VAM
4.8.1. Effect of soil temperature on VAM spore population androot colonisation in sorghum
Fig.9. Total VAM spore count in forest and rubber soil in.different locations
4.8.2. Effect of soil moisture on VAM spore population and rootcolonisation in sorghum.
4.8.3. Effect of soil organic matter on VAM colonisation insorghm and spore population in soil
Fig. 10 Effect of temperature on VAM spore count in soil
Fig. 11 Effect of temperature onVAM colonisation in roots of HM
Fig. 12. Effect of moisture on root colonisation by VAMfungi in sorghum
Fig. 13. Effect of moisture on VAM spore count in soil
4.9. Effect of cultural operations on VAM
4.9.1. Effect of fertilizers on per cent root infection and sporepopulation in Hevea
4.9.2. Effect of pfPnt protection chemicals on VAM
4.9.2.1. Effect of fungicides on V4M root colonisation in Hevea roots andspore population in soil
Fig. 14. Effect of fertilizer on VAM Spore count in soil
Fig. 15. ERect of fertilizer on VAM colonisation in HNea
4.9.2.2. Effect of insecticides on root colonisation in Hevea roots and sporepopulation in soil
4.9.2.3. Effect of weedicides on VAM colonisation in Hevea roots and sporepopulation in soil
Fig. 16. Effect of insecticides on root colonisation by VAMfungi in Hevea
Fig. 17. Effect of insecticides on VAM spore count in soil
4.9.3. Effect of solaris&itm on VAM root colonisation and sporepopulation in sorghun~
4.10. Influence of various agents on the dissemination ofVAM spores
5 DISCUSSION
5.1. Distribuaion of VAM in rubber growing soils
5.2. Influence of location and season on VAM spore population
5.3. Influence of location and season on root colonisation by VAM in Hevea
5.4. Effect of soil pH and organic carbon on VAM spore population and root colonisation in Hevea
5.5. VAM colonisation and spore population as influenced by duration and cycle of Hevea cultivation
5.6. Vertical Distribution of VAM spores in soil
5.7. Comparative study on VAM population in Hevea plantation and nearby forest
5.8. Effect of environmental factors on VAM spore count and root colonisation
5.8.1. Temperature
5.8.2. Moisture
5.8.3. Organic matter
5.9. Cultural practices on VAM spore population and root colonisation
5.9.1. Fertilizer
5.9.2. Fungicides
5.9.3. Insecticides
5.9.4. Weedicides
5.9.5. Effect of Solarisation
5.10. Dissemination of VAM
5.10.1. Dissemination by biotic agents
5.10.1.1.Earthworms
5.10.1.2. Termites
5.10.1.3. Wasps
5.10.1.4. Ants
5.10.2. Abiotic factors in the dispersal of VAM fungi
6 SUMMARY
REFERENCES