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  • Title
  • DEDICATION
  • CERTIFICATE
  • DECLARATION
  • ACKNOWLEDGEMENT
  • LIST OF TABLES
  • LIST OF FIGURES
  • LIST OF PLATES
  • CONTENTS
  • 1. Introduction
  • Bufo melanostictus
  • Diagnosis
  • Review of earlier work done
  • Justification of the present work
  • 2. Status, Habitat & Distribution
  • Study area
  • Topography and Climate
  • 1. Meterological data of the study area
  • Distribution
  • Habitat
  • 1. Map of Pathanamthitta District
  • Sexual dimorphism
  • Size
  • Secondary Sexual characters
  • Sex ratio
  • Present status of B. melanostictus
  • 3. Food and feeding habits
  • Introduction
  • Matrials and Methods
  • Results
  • 2. Stomach contents of B.melanostictus expressed as percentage of total body weight with respect to sex and month
  • 3. Food spectrum of Bufo melanostictus
  • 4. Seasonal variation in the food item of B. melanostictus
  • 5. Percentage of food items recovered from the stomach of B. melanostictus during Jan 1991 to Dec. 1992
  • 6. Monthly variation in the feeding activity of B. melanostictus
  • 2. Annual consumption of food items by B. melanostictus
  • 7. Correlation metrics of feeding activity of B. melanostictus
  • 8. Economically important organisms recovered from the gut of B. melanostictus
  • DISCUSSION
  • 4. Male reproductive cycle
  • Introduction
  • Materials and Methods
  • Results
  • Gonadosomatic index
  • Relative testicular weight
  • 9. Seasonal variation in the GSI, relative testicular weight, testis diameter, Testis tubule diameter, Ley dig cell nuclear diameter and fat body weight of B. melanostictus
  • 3. Seasonal changes in the GSI, diameter of the testis, seminiferous tubule, Leydig cell nuclear diameter and fat body weight of B. melanostictus in relation to temperature, humidity and rainfall
  • Testis diameter
  • Seminiferous tubule diameter
  • Leydig cell nuclear diameter
  • Fat body cycle
  • 10. Correlation metrics between testicular activity of B. melanostictus
  • Spermatogenetic stages
  • 11. Correlation metrics between testis diameter, tubule diameter and Leydig cell nuclear diameter of B. melanostictus
  • 12. Seasonal variation in the spermatogenetic stages of B. melanostictus
  • 1. Photomicrographs of sections of testis of B. melanostictus
  • 4. Seasonal variation in spermatogenetic stages of B. melanostictus
  • Dicussion
  • 5. Female reproductive cycle
  • Introduction
  • Materials and methods
  • Results
  • Body weight
  • Gonadosomatic Index
  • Ovarian cycle
  • 2. Photomicrographs of sections of ovary of B. melanostictus
  • 13. Seasonal variation in the ovarian activity of B. melanostictus
  • 5. Seasonal variation in the fat body weight, ovary weight, oviduct weight and GSI of B.melanostictus
  • 6. Seasonal variation in the diameter of largest oocyte of B. melanostictus
  • 1. Pre-breeding or preparatory phase
  • 2. Breeding phase
  • 3. Post breeding phase
  • Oviduct cycle
  • Fat body cycle
  • 14. Correlation co-efficient between body weight, ovary mass and various factors of ovarian activity of B. melanostictus
  • DISCUSSION
  • 6. Effects of some pesticides on the hatching rate, survival and growth of tadpoles
  • Introduction
  • Materials and methods
  • 15. List of pesticides used in the present study
  • Results
  • 16. Effect of endosulfan on the hatching rate of B. melanostictus
  • 17. Effect of hinosan on the hatching rate of B. melanostictus
  • 7. Effect of endosulfan on the mortality of tadpoles of B. melanostictus
  • 8. Effect of hinosan on the mortality of tadpoles of B. melanostictus
  • 18. 96 hr LC50, 95% confidence limits, safe concentration, slope value and regression equation for tadpoles of Bs melanostictus exposed to pesticides
  • 9. Effect of endosulfan on larval survival of B. Melanostictus
  • 10. Effect of hinosan on larval survival B. Melanostictus
  • 19. Effect of endosulfan on the weight and length of tadpoles of B. melanostictus
  • 20. Effect of hinosan on the weight and length of tadpoles of B. melanostictus
  • 11. Effect of endosulfan on the percentage of length decrease of tadpoles from their control values
  • 12. Effect of endosulfan on the percentage of weight decrease of tadpoles from their control values
  • 13. Effect of hinosan on the percentage of length decrease of tadpoles from their control values
  • 14. Effect of hinosan on the percentage of weight decrease of tadpoles from their control values
  • 21. Effect of pesticides on the percentage of length and decrease of tadpoles from their control values
  • 22. Effect of pesticides on the percentage of weight decrease of tadpoles from their control values
  • DISCUSSION
  • 7. Histopathological changes in the gonad, liver and kidney of adults exposed to sublethal concentrations of some pesticides
  • Introduction
  • Materials and methods
  • Results
  • 15. Effect of endosulfan on the mortality of adult B. melanostictus
  • 16. Effect of hinosan on the mortality of adult B. melanostictus
  • Testis
  • 23. 24 hr LD50, fiducial limits, slope value and regression equation for B.melanostictus adults exposed to pesticides
  • 24. Summerised account of histopathological effects produced by sublethal concentrations of endosulfan and hinosan in different organs of B. melanostictus
  • Exposure to 0.24 ppm/gm body weight of endosulfan
  • Exposure to 0.48 ppm/gm body weight of endosulfan
  • 3. Photomicrographs of sections of testis of B.melanostictus exposed to endosulfan (x 250)
  • Exposure to 0.054 ppm/gm body weight of hinosan
  • Exposure to 0.108 ppm/gm body weight of hinosan
  • 4. Photomicrographs of sections of testis of B. melanostictus exposed to hinosan (x 250)
  • 25. Effect of endosulfan on spermatogenetic stages of B. melanostictus
  • 26. Effect of hinosan on spermatogenetic stages of B.melanostictus
  • 17. Effect of endosulfan on the spermatogenetic stages (0 and I) of B. melanostictus
  • 18. Effect of endosulfan on the spermatogenetic stages (II and III) of B.melanostictus
  • 19. Effect of endosulfan on the spermatogenetic stages (IV and V) of B. melanostictus
  • 20. Effect of hinosan on the spermatogenetic stages (0 and I) of B. melanostictus
  • 21. Effect of hinosan on the spermatogenetic stages (II and III) of B. melanostictus
  • 22. Effect of hinosan on the spermatogenetic stages (IV and V) of B.melanostictus
  • Ovary
  • Exposure to pesticides
  • 5. Photomicrographs of sections of ovary of B.melanostictus exposed to pesticides (x 100)
  • Gonadosomatic Index
  • 27. Effect of endosulfan on the GSI of male B.melanostictus
  • 23. Effect of endosulfan on the GSI of male B. melanostictus
  • 24. Effect of endosulfan on the GSI of female B. melanostictus
  • 28. Effect of endosulfan on the GSI of female B. melanostictus
  • 29. Effect of hinosan on the GSI of male B.melanostictus
  • 25. Effect of hinosan on the GSI of male B. melanostictus
  • 26. Effect of hinosan on the GSl of female B. melanostictus
  • Liver
  • 30. Effect of hinosan on the GSI of female B. melanostictus
  • Exposure to 0.24 ppm/gm body weight of endosulfan
  • Exposure to 0.48 ppm/gm body weight of endosulfan
  • 6. Photomicrographs of sections of liver of B.melanostictus exposed to endosulfan (x 250)
  • Exposure to 0.054 ppm/gm body weight of hinosan
  • Exposure to 0.108 ppm/gm body weight of hinosan
  • 7. Photomicrographs of sections of liver of B. melanostictus exposed to hinosan (x 250)
  • Hepatosomatic Index
  • 31. Effect of endosulfan on the HSI of B.melanostictus
  • 32. Effect of hinosan on the HSI of B. melanostictus
  • 27. Effect of endosulfan on the HSI of BR melanostictus
  • 28. Effect of hinosan on the HSI of B. melanostictus
  • Kidney
  • Exposure to 0.24 ppm/gm body weight of endosulfan
  • Exposure to 0.48 ppm/gm body weight of endosulfan
  • 8. Photomicrographs of sections of kidney of B. melanostictus exposed to endosulfan (x 250)
  • Exposed to 0.054 ppm/gm body weight of hinosan
  • Exposed to 0.108 ppm/gm body weight of hinosan
  • 9. Photomicrographs of sections of kidney of Bs melanostictus exposed to hinosan (x 250)
  • Dicussion
  • 8. Summary and Conclusions
  • SUMMARY
  • CONCLUSlON
  • 9. References