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Full Screen- Title
- DEDICATION
- CERTIFICATE
- DECLARATION
- ACKNOWLEDGEMENT
- CONTENTS
- 1. Introduction and Review of Literature
- Risk Factors
- Sugar Cataracts
- Sunlight and UV Cataract
- lonising Radiation and Cataract
- Smoke, Smoking and Cataract
- Nutritional Status and Cataract
- Diarrhoea and Cataract
- Blood Pressure and Cataract
- Toxic Chemicals, Drugs, Medical Treatments and Cataract
- Trace Elements and Cataract
- Selenium and the Living World
- Selenite Cataract
- 2. Materials and Methods
- Part l. Cataractogenic Potency of Sodium Selenite
- Age-Dependent Effects
- Dosage-Dependent Effect
- Anti-Cataractogenic Potency of Vitamin-E
- Part II. Histomorphological Studies
- Cataract Induction
- Light Microscopy
- Materials needed
- Procedure
- Staining Method (Haemotoxylin-Eosin)
- Scanning Electron Microscopy
- Materials Needed
- Procedure
- Part Ill. Biochemical Studies
- Estimation of Protein
- Estimation of Glutathione
- Estimation of Sulfhydryl Groups
- Estimation of Total Sutfhydryl Groups (TSH)
- Estimation of Non-Protein Sutfhydryl Groups (NPSH)
- Determination of Protein Bound Sulfhydryl Groups (PSH)
- Estimation of Ascorbic Acid
- Estimation of Nucleic Acids
- Estimation of Ribonucleic Acid (RNA)
- Estimation of Deoxyribonucleic Acid (DNA)
- Estimation of Glucose
- Estimation of Fructose
- Estimation of Thiobarbituric Acid (TBA) Reactive Material or Malondialdehyde (MDA)
- Determination of Potassium and Sodium
- Determination of Calcium and Magnesium
- Assay of Adenosine Triphosphatase (ATPase) (E.C. 3.6.1.3) Activity
- Determination of Inorganic Phosphate
- Assay of Acid Phosphatase (ACP) (E.C.3.1.3.2)
- Assay of Glucose-6-Phosphate Dehydrogenase (G-6-PDH) (E.C.1.1.1.49) Activity
- Assay of Succinic Dehydrogenase (SDH) (E.C.1.3.98.1) Activity
- Assay of y-Glutamyl Cysteine Synthetase (y-GCS) (E.C.6.3.2.2)
- Assay of Glutathione Reductase (GSH-R) (NADPH: OxidisedGlutathione Oxidoreductase) (E.C.1.6.4.2)
- Determination of Water Contents
- Statistical Analysis
- Part lV. Electrophoretic Studies
- Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS PAGE)
- Preparation of Slab Gels
- Sample Preparation and Sample Loading
- Silver Staining Method
- 3. Observation and Results
- Part l. Cataractogenic Potency of Sodium Selenite
- Age-Dependent Effect
- Dosage-Dependent Effect
- Anti-Cataractogenic Potency of Vitamin-E
- Table 1. Cataractogenic potency of sodium selenite with multiple low (chronic) dosages in different age groups (age-dependent effect)
- Table 2. Cataractogenic potency of sodium selenite with multiple and single high (acute) dosages in different age groups (age-dependent effect)
- Table 3. Cataractogenic potency of sodium selenite with multiple low (chronic) dosages in 8 day post partum rat pups (dosage-dependent effect)
- Table 4. Cataractogenic potency of sodium selenite with multiple and single high (acute) doses in 10 day post partum rat Pups (dosage-dependent effect)
- Table 5. Proportionality of minimum dosage requirement of sodium selenite to produce cataract with low (chronic) and high (acute) dosages
- Table 6. Anti-cataractogenic potency of vitamin-E
- Part II. Histomorphological Studies
- Light Microscopy
- Scanning Electron Microscopy
- Diagrammatic representation of the anterio-posterior section of the lens
- Plate I. Photographs of nuclear cataract produced by multiple low dosage group and the vitamin-E treated lenses
- Plate II. Wotographs of different stages of cataract produced by acute high dosage groups
- Plate III. Light Microscopy
- Plate IV. Light Microscopy
- Plate V. Light Microscopy
- Plate VI. Scanning Electron Microscopy
- Plate VII. Scanning Electron Microscopy
- Plate VIIl. Scanning Electron Microscopy
- Plate IX. Scanning Electron Microscopy
- Plate X. Light Microscopy
- Plate XI. Scanning Electron Microscopy
- Part Ill. Biochemical Studies
- Protein
- Glutathione
- Sulfhydryl Groups
- Ascorbic Acid
- DNA and RNA
- Glucose and Fructose
- Thiobarbituric Acid Reactive Material (Malondialdehyde)
- Sodium, Potassium, Magnesium and Calcium
- Adenosine Triphosphatase
- Acid Phosphatase
- Glucose-6-Phosphate Dehydrogenase
- Succinic Dehydrogenase
- y-Glutamyl Cysteine Synthetase
- Glutathione Reductase
- Lens Weight and Water Contents
- Part IV. Electrophoretic Studies
- Plate XII. SDS PAGE
- Plate XIII. SDS PAGE
- Plate XIV. SDS PAGE
- 4. Discussion
- Part l. Cataractogenic Potency of Sodium Selenite
- Age-Dependent Effect
- Dosage-Dependent Effect
- Anticataractogenic Potency of Vitamin-E
- Part II. Histomorphological Studies
- Light Microscopy
- Scanning Electron Microscopy
- Part Ill. Biochemical Studies
- Proteins
- Glutathione
- Sulfhydryl Groups
- Ascorbic Acid
- DNA and RNA
- Glucose and Fructose
- Malondialdehyde
- Sodium, Potassium, Magnesium and Calcium
- Adenosine Triphosphatases
- Acid Phosphatase
- Glucose 6-Phosphate Dehydrogenase
- Succinic Dehydrogenase
- y-Glutamyl Cysteine Synthetase
- Glutathione Reductase
- Lens Weight and Water Content
- Part IV. Electrophoretic Studies
- 5. Summary and Conclusion
- BIBILIOGRAPHY