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TITLE
DEDICATION
DECLARATION
CERTIFICATE
ACKNOWLEDGEMENT
PREFACE
ABSTRACT
ABBREVIATIONS
CONTENTS
LIST OF FIGURES
LIST OF TABLES
LIST OF PLATES
1. INTRODUCTION
Causes of cancer
Aims and objectives
2. REVIEW OF LITERATURE
2.1 Cytotoxic screening models
2.2. Tumour models for testing antitumour drugs
2.3 Primary screening of plant compounds for anticancer activity in mice tumour models
2.4 Oxidants, anti-oxidants and carcinogenesis
2.5 Chemical Carcinogenesis an overview
2.6 Chemical Carcinogenesis models
2.7 Chemopreventive properties of medicinal plants
2.8 Apoptosis, the programmed cell death
2.9 Apoptosis and cancer chemotherapy
2.10 About the plant
3. MATERIALS AND METHODS
3.1 Preparation of aqueous extract of medicinal plants
3.2 In vitro screening of medicinal plants for cytotoxicity
3.3 Screening of organic extracts of Cassia fistula L for cytotoxicity
3.4 Long Term cytotoxicity assay by MTT Assay
3.5 Antitumour effect of methanolic extract of Cassia fistula L. (MEC)
3.6 Murine two stage skin chemical carcinogenesis
3.7 Apoptogenic potential of MEC
3.8 Statistical Analysis
4. RESULTS
4.1 Short term in vitro cytotoxicity screening of medicinal plants
4.2 Short term in vitro cytotoxicity screening of different organic extracts of the bark of Cassia fistula L.
4.3 Cytotoxic screening of MEC by MTT assay
4.4 Antitumour effect of methanolic extract of Cassia fistula L. in Balb/c mice
4: 5 Chemo preventive potential of MEC of C fistula L.
4.6 Apoptogenic potential of MEC
5. DISCUSSION
5.1 Short term in vitro cytotoxicity screening of medicinal plants
5.2. Short term in vitro cytotoxicity screening of different organic extracts of the bark of Cassia fistula L.
5.3 Cytotoxic screening of MEC by MTT assay
5.4 Antitumour effect of methanol extract of Cassia fistula L. inBalb/c mice
5.5 Chemo preventive potential of MEC of C. fistula L.
5.6 Apoptogenic effect of C. fistula L.
6. SUMMARY AND CONCLUSION
7. BIBLIOGRAPHY