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Full Screen- TITLE
- DEDICATION
- CERTIFICATE 1
- CERTIFICATE-2
- DECLARATION
- CONTENTS
- LIST OF TABLES
- LIST OF FIGURES
- ABBREVIATIONS
- I. INTRODUCTION
- Definition and Nomenclature of Metallothioneins
- Biosynthesis
- Localization
- Structure
- Importance of Eichhornia crassipes in Metal Detoxification
- Summary of the aims
- II. REVIEW OF LITERATURE
- Plants Heavy Metal Uptake Strategy
- Heavy Metals in FVPs
- Defensive Strategy of Plants
- Phytochelatins
- PCs Have the General Structure (γ-EC) n-G
- PCs are Synthesised from GSH
- PC Synthase is Activated by Heavy Metal Ions
- PC Biosynthesis may be Regulated in Several Ways
- PC-Cd Complexes are Sequestered in Vacuoles
- Sulphide Ions Play a Role in PC Function
- PC Isolation, Detection and Quantitation
- III. BIOCHEMICAL INVESTIGATIONS
- MATERIALS AND METHODS
- Estimation of Protein - Lowrys Method (Lowry1951)
- Estimation of Total Thiols (Jocelyn, 1991)
- Estimation of GSH: - Ellmans Method (Ellman, 1959)
- Atomic Absorption Spectroscopy (AAS)
- RESULTS
- GENERAL DISCUSSION
- IV. PURIFICATION OF DIFFERENT PHYTOCHELATINS
- 4.1 PURIFICATION OF Cd EXPOSED PEPTIDES
- Step-1: Extraction
- Step-2: Ion-Exchange Chromatography
- Step 3: Salting out and Concentration
- Step 4: Gel Filtration
- Estimation of Total Thiols (Jocelyn, 1991)
- Atomic Absorption Spectroscopy (AAS)
- RESULTS
- 4.2 PURIFICATION OF Cu EXPOSED PEPTIDES
- Step-1: Extraction
- Step-2: Ion-Exchange Chromatography
- Step 3: Salting out and Concentration
- Step 4: Gel Filtration Estimation of Total Thiols (Jocelyn, 1991)
- Atomic Absorption Spectroscopy (AAS)
- RESULTS
- 4.3 PURIFICATION OF Zn EXPOSED PEPTIDES
- Step-1: Extraction
- Step-2: Ion - Exchange Chromatography
- Step 3: Salting out and Concentration
- Step 4: Gel Filtration Estimation of Total Thiols (Jocelyn, 1991)
- Atomic Absorption Spectroscopy (AAS)
- RESULTS
- GENERAL DISCUSSION
- V HPLC FOR PHYTOCHELATINS
- MATERIALS AND METHODS
- Instrumentation
- Sample Preparation
- RP-HPLC Procedure
- Gradient Elution
- RESULTS
- DISCUSSION
- VI IN VITRO SYNTHESIS OF PHYTOCHELATINS
- Enzyme Preparation
- In Vitro Studies
- RESULTS
- DISCUSSION
- VII STUDIES ON PURIFIED PHYTOCHELATINS
- 7.1 pH ANALYSIS OF PCs
- MATERIALS AND METHODS
- DISCUSSION
- 7.2 IMMOBILISATION OF PEPTIDES AND METAL ION SUBSTITUTION
- MATERIALS AND METHODS
- Procedure
- Atomic Absorption Spectroscopy (AAS)
- RESULTS
- INFERENCES AND DISCUSSION
- VIII SUMMARY
- BIBLIOGRAPHY