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Full Screen- TITLE
- DEDICATION
- CERTIFICATE
- DECLARATION
- ACKNOWLEDGEMENT
- ABBREVIATIONS
- CONTENTS
- 1 INTRODUCTION
- The Objectives of this Study
- 2 REVIEW OF LITERATURE
- 3 MATERIALS AND METHODS
- 3.1 Galactose Cataract
- I. Control group
- II. Experimental group
- Ill. Vitamin E Supplemented group
- 3.2 Selenium Cataract
- 3.3 Preparation of lens extract
- 3.4 Collection and preservation of blood
- Processing of blood samples (Beutler, 1975)
- (a) Preparation of packed red cell
- (b) Preparation of 1: 20 hemolysate
- 3.5 BIOCHEMICAL ANALYSIS
- Hemoglobin (Beutler, 1975)
- Protein (Lowry 1951)
- Superoxide dismutase (EC 1.15.1.I) (Paoletti and Mocali, 1990)
- Reduced glutathione (Beutler. 1986)
- Glutathione reductase (ED 1.6.4.2) (Beutler, 1986)
- Glutathione-S-transferase (EC 2.5.1.45) (Beutler, 1986)
- Malondialdehyde: - (John A Buege and Stevan P. Aust 1978)
- 3.6 Glycoprotein
- 3.7 Sodium Dodesyl Sulphate polyacrylamide gel electrophoresis (SDS-PAGE) (Laemle 1970)
- 3.8 Statistical Analysis
- 4 RESULTS
- Fig. 4.2.A Mean wet weight of lens (mg) in normal, cataractous and vitamin E supplemented rats in galactose induced cataract
- Fig. 4.2.B Mean wet weight of lens (mg) in normal, cataractous and vitamin C supplemented rats in selenium induced cataract
- Plate. 4.1.A. Control lens
- Plate 4.1.B. Stage 1. Galactose cataract (1 0 days)
- Plate. 4.1.C. Stage 2. Galactose cataract (20 days)
- Plate. 4.1.D. Stage 3. Galactose cataract (30 days)
- Plate.4.1.E. Selenium cataract after one month
- 4.3 Various stages of preventive effect of vitamin E on galactose cataract
- Plate.4.3.A. Stage 1. (10 days)
- Plate.4.3.B. Stage 2. (20 days)
- Plate. 4.3.C. Stage 3. (30 days)
- Plate. 4.3 D. Preventive effect of vitarninc on selenium cataract after one month
- 4.4 Oxidative Stress
- 4.5 Lipid Peroxidation
- 4.6 Glycoprotein
- Plate 4.7.A Protein Profile of Galactose Induced Cataract affected Lens from 10 days old rat
- 4.7 Electrophoresis
- Plate 4.7.B Protein Profile of Galactose Induced Cataract affected Lens from 20 days old rat
- Plate 4.7.C Protein Profile of Galactose Induced Cataract affected Lens from 30 days old rat
- Plate 4.7.D Protein Profile of Selenium Induced Cataract affected Lens After One Month old rat
- 5 DISCUSSION
- 5.1 Lens Weight
- 5.2 Oxidative stress
- 5.3 Lipid Peroxidation
- 5.4 Glycoprotein
- 5.5 Effect of VitaminE and Vitamin C on Cataract
- 5.6 Electrophoresis
- 6 SUMMARY AND CONCLUSION
- REFERENCE