Online Thesis Search Results

TITLE
DECLARATION
CERTIFICATE
ACKNOWLEDGEMENT
CONTENTS
LIST OF TABLES
LIST OF ILLUSTRATIONS
ABBREVIATIONS
1. Introduction and Review of Literature
1.1 Introduction
1.2 REVIEW OF LITERATURE
1.2.1 Etiology of cancer
1.2.2 Attributes of transformed cells
1.2.3 Biological aspects of cancer cells
Dlfferentlatlon and anaplasla
Rate of Growth
Encapsulation - lnvasion
Metastasis
1.2.4 Immunology of tumour cells
Tumour antigens
Evaslon of speclflc tumour lmmunlty
1.2.5. Interaction between neoplastic cells and the hosts lmmunoreyuliitory apparatus
1.2.6 Immunotherapy
Active nonspeclflc lmmunotherapy
Mlcroblal augmenting agents
Synthetic rnacrophage activators and Interferon Inducers.
Cytokines
Adoptive lmmunotherapy
Active Specific immunotherapy
Passlve lmmunotherapy
Immunomodulators
lmmunomodulators of plant origin
1.1 Plant immunomodulators with anticancer activity
Role of immunomodulators in cancer therapy
1.2.7 Lectins
1.2.8 Plants and plant products in cancer therapy
Plants of Loranthaceae famlly
HELICANTHES ELASTICA (Famlly - Loranthaceae)
1.1 Helicanthes elastica
2. Materials and Methods
2.1 MATERIALS
2.1.1 Plant
2.1.2 Chemicals
2.1.3 Instruments
2.1.4 Cell Lines
2.2 METHODS
2.2.1 Maintenance of experimental animals
2.2.2 Tumour cell maintenance
2.2.3 Determination of in vitro cytotoxicity
2.2.4 Tissue Culture - Maintenance of Cell Line
2.2.5 Determination of cytotoxicity using tissue culture
2.2.6 Anti-tumour testing using murine model
2.2.7 Determination of Haemagglutination
2.3 BIOCHEMICAL ESTIMATION
2.3.1 Determination of Protein
2.3.2 Estimation of DNA Synthesis
2.3.3 Estimation of RNA synthesis
2.3.4 Estimation of Protein synthesis
2.3.5 Determination of Haemoglobin
2.3.6 Determination of total WBC and DC
2.3.7 Determination of alkaline phosphatase activity in mice liver homogenateand serum
2.3.8 Determination of glutamate pyruvate transaminase in mice liverhomogenate or serum
2.3.9 Sodium Dodecyl Sulphate (SDS) Polyacrylamide gelelectrophoresis
2.3.10 Amino Acid Determination
2.3.11 Determination of Sugar (Dubois Method)
2.4 IMMUNOLOGICAL ASSAYS
2.4.1 Assay for circulating antibody titre
2.4.2 Jernes plaque assay
2.4.3 Macrophage mediated cytotoxicity assay
2.4.4 Lymphocyte Proliferation assay - in vitro and in vivo
2.4.5 Assay for Natural Killer cell activity (NK, activity)
2.4.6 Assay for Antibody Dependant Cellular Cytotoxicity (ADCC)
2.4.7 Antibody - Complement mediated cytotoxicity (ACC) assay
2.5 OTHER PARAMETERS
2.5.1 Determination of Bone Marrow Cellularity
2.5.2 Histological Examination
2.6 STATISTICAL EVALUATION
3. Cytotoxic and antitumour activity of Helicanthes elastica
3a CYTOTOXIC AND ANTITUMOUR ACTIVITY
3a.1 MATERIALS AND METHODS
3a.2 RESULTS
3.1 Cytotoxicity of the Crude extracts of H.E. grown on different host trees to EAC cells in vitro
3.2 Cytotoxicity of the extract of H.E. (Host tree - Mangifera indica) to EAC tumour cells in vitro after various treatments
3.1 Elution profile of cytotoxic fractions from H.E. on sephadex G-50 (Host tree - Mangifera indica)
3.2 Elution profile of cytotoxic fractions from H.E. through sephadex G-50 (Host tree - Citrus maxima)
3.3 Elution profile of partially purified extract of H.E. through DE 52 column (Host tree - Mangifera indica)
3.4 Elution profile of partially purified extract of H.E. through DE 52 column (Host tree - Citrus maxima)
3.3 Cytotoxicity of.the partially purified extract of H.E. to various cells in vitro (Host tree - Mangifera indica)
3.4 Cytotoxicity of the partially purified extract of H.E. to various cells in vitro (Host tree - Citrus maxima)
3.5 Effect of H.E. extracts on growth inhibitory study of L-929 cells in culture.
3.5 Cytotoxicity of intraperitoneal administration of the extract of H.E. on ascites tumour reduction.
3.6 Effect of prophylactic administration of partially purified extracts of H.E. on solid tumour induced by DLA cells
3.7 Effect of partially purified extracts of H.E. on developed solid tumour induced by DLA cells.
3.8 Effect of simultaneous administration of partially purified extracts of H.E. on solid tumour induced by DLA cells.
3a.3 DISCUSSION
3.9 Effect of simultaneous administration of partially purified extracts of H.E. on solid tumour induced by EAC cells.
3b. TOXICITY STUDIES
3b.1 MATERIALS AND METHODS
3b.2 RESULTS AND DISCUSSION
3.10 Effect of administration of the extract of H.E. on body weight of mice
3.6 Effect of partially purified extract of H.E. on liver and serum GPT.
3.7 Effect of partially purified extract of H.E. on liver and serum ALP
4. Characterization of the anti-tumour principle from the extract of Helicanthes elastica
4.1 MATERIALS AND METHODS
4 2 RESULTS
4.1 Molecular sieve chromatography of H.E. extract (Host tree - Mangifera indica)
4.2 UV absorbance of partially purified extract of H.E. at different wavelengths
4.1 Effect of the heat, acid and DTT on the cytotoxicity of partially purified extract of H.E.
4.2 Reversal of cytotoxicity of the extract of H.E. using MLI antibody
4.3 Sugar specificity of the partially purified extract of H.E. using EAC cells
4.3 Inhibition of cytotoxicity of the partially purified extract of H.E. with various dilutions of ascites fluid and cell sonicate
4.4 Effect of partially purified extracts of H. E. on 3H thymidine incorporation into EAC cells
4.5 Effect of partially purified extracts of H.E. on 3H leucine Incorporation into protein.
4.6 Effect of partially purified extracts of H. E. on 3H uridine incorporation into RNA.
4.7 Polyacrylamide gel electrophoresis pastern of the partially purified material of H.E.
4.8 Molecular weight determination of active principle from H.E. using known molecular weight standards
4.9 HPLC chromatogram of the active principle from H.E. extract.
4.4 Amino acid analysis of the active protein isolated from H.E.
4.3 DISCUSSION
5. Immunomodulatory activity of Helicanthes elastica
5.1 MATERIALS AND METHODS
5.2 RESULTS
5.1 Effect of the extract of H.E. on WBC count
5.2 Effect of the extract of H.E. on polymorphonuclear leukocytes
5.1 Effect of administration of the partially purified extract of H.E. on body weight and haemoglobin level of mice
5.2 Effect of partially purified extract of H.E. on weight of secondary lymphoid organs
5.3 Effect of partially purified extract of H.E. on circulating antibody titre
5.3 Effect of the extract of H.E. on plaque forming cells
5.4 Effect of treatment of H.E. extract on lymphocyte proliferation.
5.4 Effect of the extract of H.E. on macrophage mediated cytotoxicity in vivo
5.5 Effect of the extract of H.E. on macrophage mediated cytotoxicity in vitro
5.6 NK-cell activity of spleen cells treated with partially purified extract of H.E.
5.3. DISCUSSION
5.7 ADCC - activity of spleen cells treated with partially purified extract of H. E.
5.8 Effect of the extract of H.E. on antibody complement mediated cytotoxicity.
6. Radioprotective and chemoprotective action of Helicanthes elastica
6.1 MATERIALS AND METHODS
6.2 RESULTS
6.1 Effect of partially purified extract of H.E. on WBC count of irradiated mice (400 rads)
6.2 Effect of partially purified extract of H E. on the haemoglobin level of irradiated mice (400 rads)
6.1 Effect of partially purified extract of H.E. on BM cellularity of Irradiated mice
6.3 Effect of partially purified extract of H.E. on the body weight of irradiated mice (400 rads)
6.2 Effect of H.E. on liver GPT and ALP of irradiated mice.
6.4 Pathological studies on intestine of irradiated mice with and without treatment with H.E. extract.
6.5 Effect of partially purified extract of H.E. on WBC count of animals treated with CYP.
6.6 Effect of partially purified extract of H.E. on polymophonuclear cells of animals treated with GYP.
6.7 Effect of partially purified extract of H.E. on haemoglobin level of mice treated with GYP.
6.3 Effect of partially purified extract of H.E. on the survival of animals, treated with CYP
6.3 DISCUSSION
6.4 Effect of partially purified extract of H.E. on the body weight of animals treated with CYP
7. Isolation and biological activity of a lectin from Helicanthes elastica
7.1 MATERIALS AND METHODS
7.2. RESULTS
7.1 Elution profile of cytotoxic fractions from H.E. extract on sephadex G-50 (Host tree Mangifera indica)
7.1 In vitro cytotoxicity of the partially purified lectin isolated from H.E.
7.2 Effect of partially purified lectin from H.E on Ehrlich ascites tumour reduction.
7.2 Effect of administration of lectin on solid tumour reduction induced by DLA cells.
7.3 Sugar specificity of the partially purified lectin
7.4 Reversal of cytotoxicity of the partially purified lectin using MLI antibody.
7.3 DISCUSSION
8. Summary and Conclusion
BIBILIOGRAPHY