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TITLE
DECLARATION
DEDICATION
CERTIFICATE
ACKNOWLEDGEMENT
CONTENTS
I. INTRODUCTION
II. MATERIALS AND METHODS
C. FOLIAR EPIDERMAL STUDIES
A. GERMINATION STUDIES
Clearing techniques used for study of palisade ratio.
B. HISTOCHEMICAL ANALYSIS - QUALITATIVE AND QUANTITATIVE
1. Insoluble polysaccharides
2. Sulphated and Carboxylated polysaccharides
3. starch
4. Total Proteins
1. Chlorophyll & Carotenoid contents
2. Total Carbohydrate content
Preparation of Standard Carbohydrate Solution
4. Total protien
Reagents for Lowry Method
D. MORPHOLOGICAL STUDIES
E. PHYTOMASS AND PRODUCTIVITY
F. YIELD STUDIES
G. SCANNING ELECTRON MICROSCOPIC STUDIES OF SEEDS - (SEM)
III. OBSERVATIONS
A. GERMINATION STUDIES
a. Germination percentage
b. Radicle and hypocotyle length
B. HISTOCHEMICAL ANALYSIS
a. Qualitative
Plate 1. Histochemical localisation of starch in foliar epidermis I2KI reaction Capsicum Frutescens L.
Plate 2. Histochemical localisation of starch in foliar epidermis I2KI reaction Solanum Melongena L.
Plate 3. Histochemical localisation of starch in foliar epidermis I2KI reaction Solanum Nigrum L.
Plate 4. Histochemical localisation of insoluble polysaccharides in foliar epidermis (PAS reaction) Capsicum Frutescens L.
Plate 5. Histochemical localisation of insoluble polysaccharides in foliar epidermis (PAS reaction) Solanum Melongena L.
Plate 6. Histochemical localisation of insoluble polysaccharides in foliar epidermis (PAS reaction) Solanum Nigrum L.
Plate 7. Histochemical localisation of sulphated and carboxylated polysaccharides in foliar epidermis (Toludine Blue O Reaction) Capsicum Frutescens L.
Plate 8. Histochemical localisation of sulphated and carboxylated polysaccharides in foliar epidermis (Toludine Blue O Reaction) Solanum Melongena L.
Plate 9. Histochemical localisation of sulphated and carboxylated polysaccharides in foliar epidermis (Toludine Blue O Reaction) Solanum Nigrum L.
Plate 10. Histochemical localisation of protein in Foliar epidermis mercuric bromophenol blue reaction Capsicum Frutescens L.
Plate 11. Histochemical localisation of protein in Foliar epidermis mercuric bromophenol blue reaction Solanum Melongena L.
Plate 12. Histochemical localisation of protein in Foliar epidermis mercuric bromophenol blue reaction Solanum Nigrum L.
b. Quantitative
C. FOLIAR EPIDERMAL STUDIES
a. Stomata indxes, Frequencies and Abnormalities
b. Palisade ratio
D. MORPHOLOGICAL STUDIES
a. Height of the plants
b. Length of the roots
c. Number of leaves
d. Number of branches
E. PHYTOMASS AND PRODUCTIVITY
a. Phytomass - Stem
b. Phytomass – Leaves
c. Phytomass - Root
d. Total phytomass
e. Net primary productivity
F. YIELD STUDIES
a. Number of flowers
b. Number of fruits
G. SEM STUDIES OF SEEDS
Plate 13. Scanning electron micrographs of the seeds of Capsicum Frutescens
Plate 14. Scanning electron micrographs of the seeds of Solanum Melongena L.
Plate 15. Scanning electron micrographs of the seeds of Solanum Nigrum
IV. DISCUSSION
GERMINATION STUDIES
Radicle a hypocotyle elongation
HISTOCHEMICAL ANALYSIS
Chlorophyll content
Carotenoid
Total Carbohydrate
Total protein content
FOLIAR EPIDERMAL STUDIES
Stomata indxes, Frequencies and Abnormalities
Palisade ratio
GROWTH, PHYTOMASS, PRODUCTIVITY AND YIELD
SEM STUDIES OF SEEDS
V. SUMMARY AND CONCLUSION
VI. LITERATURE CITED