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  • Title
  • DEDICATION
  • CERTIFICATE 1
  • CERTIFICATE-2
  • DECLARATION
  • ACKNOWLEDGEMENT
  • CONTENTS
  • List of Abbreviations
  • 1 Introduction
  • Aims and objectives of the study
  • 2 Review of Literature
  • 2.1 Storage of Fishes
  • 2.2 Storage of Fruits and Vegetables
  • 2.3 Storage of Fishery Products
  • 2.4 Storage of Meat Samples
  • 3 Materials and Methods
  • 3.1 Food Samples
  • 3.2 Spices
  • 3.3 Chemicals
  • 3.4 Experimental Design
  • 3.4.1 Storage Durations
  • 3.4.2 Biochemical Analysis
  • Determination of Moisture Content (MC)
  • Estimation of Protein
  • Extraction of Tissue for Water Soluble Protein
  • Extraction of Tissue for Salt Soluble Protein
  • Estimation of Crude Protein
  • Extraction of Tissue for Lipid Estimation
  • Estimation of 1-opal Lipids
  • Estimation of Phospholipids
  • Estimation of Triglycerides (TG)
  • Estimation of Free Fatty Acids
  • Preparation of tissue homogenate for the estimation of lipid peroxides
  • Estimation of Thiobarbituric Acid Reactive Substances (TBARS)
  • Estimation of Conjugated Dienes (CD)
  • Estimation of Superoxide Dismutase (SOD)
  • Estimation of Glutathione
  • Estimation of Lactate Dehydrogenase (LDH)
  • Estimation of Glycogen
  • 3.4.3 Statistical Analysis
  • 3.4.4 Antimicrobial property of spices on refrigerated buffalo meat
  • 4 Biochemical Changes of Food Samples During Frozen Storage
  • 4.1 Introduction
  • 4.2 Materials and Methods
  • 4.2.1 Materials
  • 4.2.2 Processirrg
  • I. Marine fishes
  • II.Fresh water fishes
  • III. Molluscs
  • IV. Meat samples
  • 4.3 Results and Discussion
  • 4.3.1 Effect of froden storage on proteins of food samples
  • 4.3.2 Lipid profile during frozen storage
  • 4.3.3 Changes in lipid peroxides
  • 4.3.4 Changes of moisture content (MC), glycogen and lactate dehydrogenase (LDH)
  • 5 Antimicrobial Effect of Plant Extracts on Minced Buffalo Meat
  • 5.1 Introduction
  • 5.2 Materials and Methods
  • 5.2.1 Materials
  • 1. ALL SPICE or PIMENTA
  • 2. CORIANDER
  • 3. CURRY LEAF
  • 4. PEPPERMINT
  • 5.2.2 Methods
  • 1. Preparation of leaf extracts
  • 2. Preparation of minced meat
  • 3. Incubation of buffalo Ineat samples in plant extracts
  • 5.3 Results and Discussion
  • 5.3.1 Antimicrobial Properties of Food Additives on Minced Buffalo Meat underChilled Temperature
  • 5.3.2 Antimicrobial Properties of Food Additives on Minced Buffalo Meatunder Frozen Temperature
  • 6 General Discussion
  • Significance of the present investigation
  • 7 Summary
  • References