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  • TITLE
  • DEDICATION
  • DECLARATION
  • CERTIFICATE
  • CONTENTS
  • I. INTRODUCTION
  • II. MATERIALS AND METHODS
  • Germination studies
  • 2. Qualitative analysis
  • 3. Quantitative analysis
  • 4. Foliar epidermal studies
  • 5. Morphological studies
  • 6. Phytomass and productivity
  • 7. Yield studies
  • 8. Scanning Electron Microscopic Study (SEM Studies)
  • Photography
  • 9.Bioaccumulation
  • III. OBSERVATIONS
  • 1. Germination studies
  • a) Germination percentage
  • b) Radicle and hypocotyl length
  • c) Vigour index
  • d) Phytotoxicity
  • 2. Qualitative analysis
  • Histochemical analysis
  • a) Starch
  • b) Insoluble polysaccharides
  • c) Sulphated and Carboxylated polysaccharides
  • d) Total proteins
  • e) Lipids
  • f) Cellulose
  • 3. Quantitative analysis
  • a) Chlorophyll
  • PLATE-I Histochemical localisation of starch in the foliar epidermis of Spilanthes acmella Murr.
  • PLATE -2 Histochemical localisation of starch in the foliar epidermisof Emilia sonchifolia DC.
  • PLATE -3 Histochemical localisation of starch in the foliar epidermisof Eclipta alba Hassk.
  • PLATE -4 Histochemical localisation of insoluble polysaccharides inthe foliar epidermis of Spilanthes acmella Murr.
  • PLATE -5 Histochemical localisation of insoluble polysaccharides inthe foliar epidermis of Emilia sonchifolia DC.
  • PLATE - 6 Histochemical localisation of insoluble polysaccharides inthe foliar epidermis of Eclipta alba Hassk.
  • PLATE -7 Histochemical localisation of carboxylated polysaccharidesand polyphenols in the foliar epidermis of Spilanthes acme/la Murr.
  • PLATE -8 Histochemical localisation of carboxylated polysaccharidesin the foliar epidermis of Eclipta alba Hassk.
  • PLATE -9 Histochemical localisation of carboxylated polysaccharidesin the foliar epidermis of Emilia sonchifolia DC.
  • PLTE -10 Histochemical localisation of protein in foliar epidermis ofSpilanthes acmella M urr.
  • PLATE -11 Histochemical localisation of protein in foliar epidermis ofEmilia sonchifolia DC.
  • PLATE -12 Histochemical localisation of protein in the foliarepidermis of Eclipta alba Hassk.
  • PLATE - 13 Histochemical localisation of lipids in the foliar epidermisof Spilanthes acmella Murr.
  • PLATE -14 Histochemical localisation of lipids in the foliar epidermisof Emilia sonchifolia DC.
  • PLATE -15 Histochemical localisation of lipids in the foliar epidermisof Eclipfa alba Hassk.
  • PLATE - 16 Histochemical localisation of cellulose in thefoliar epidermis of Eclipta a/ba Hassk.
  • b) Carotenoid
  • c) Total carbohydrate
  • d) Total proteins
  • 4. Foliar epidermal studies
  • a) Stomatal index
  • b) Stomatal frequency
  • c) Stomatal abnormality
  • d) Palisade ratio
  • 5. Morphological studies
  • a) Height of the plant
  • b) Length of the root
  • c) Number of leaves and branches
  • 6. Phytomass and Productivity
  • a) Phytomass of stem
  • b) Phytomass of leaves
  • c) Phytomass of root
  • d) Total phytomass
  • e) Net primary productivity
  • 7. Yield studies
  • a) Number of flowers
  • b) Number of fruits
  • 8. SEM Studies
  • PLATE -18 Scanning electron micrographs of the seeds ofSpilan fhes acmella M urr.
  • PLATE -19 Scanning electron micrographs of the seeds ofSpilanthes acrnella Murr. (Portion enlarged)
  • PLATE -20 Scanning electron micrographs of the seeds ofEmifia sonchifo /ia D C.
  • PLATE -21 Scanning electron micrographs of the seeds ofEmilia sonchifolia DC. (Portion enlarged)
  • PLATE -22 Scanning electron micrographs of the seeds of Eclipta alba Hassk. (Portion enlarged)
  • 9. Bioaccumulation of the heavy metals
  • IV. DISCUSSION
  • V. SUMMARY AND CONCLUSION
  • VI. REFERENCES