Online Thesis Search Results

TITLE
CERTIFICATE
DECLARATION
ACKNOWLEDGEMENT
DEDICATION
CONTENTS
LIST OF TABLES
LIST OF FIGURES
LIST OF ABBREVIATIONS
1. INTRODUCTION AND REVIEW OF LITERATURE
1.1 INTRODUCTION
1.2 REVIEW OF LITERATURE
1.2.1 Metastasis
1.2.2 Metastatic cascade
1.The metastatic cascade
1.The pathogenesis of metastasis
1.2.3 Growth rate and metastatic frequency
1.2.4 Cell surface properties of metastatic tumour cells
1.2.5 Cell attachment proteins in defining cell matrix
1.2.5 (a) Attachment Proteins
1.2.5 (b) Collagen
1.2.5 (c) Proteoglycans
1.2.6 Changes in matrix molecules and adhesion factors with cell transformation and malignancy
1.2.7 Tumour cell detachment and transport
1.2.8 Cell-cell and cell-matrix interactions during tumour metastasis
Tumour cell - endothelial cell interaction
Tumour cell-basement membrane interaction
Tumour cell - organ parenchyma cell interactions
1.2.9 Tumour cell invasion mechanisms
Tumour cell - organ invasion
Turnour cell motility
Tumour cell chemotaxis
1.2.10 Tumour cell growth mechanisms
Tumour autocrine growth factors
Tumour paracrine growth fnctors
1.2.11 Anti tumour host response mechanisms
Types of antimetastatic host effector mechanism
Suppression of antimetastatic host response
1.2.12 Basement membrane
Penetration by tumour cells
Basement membrane composition
2.Three step hypothesis for tumour cell penetration of the basement membrane.
1.2.13 Role of matrix degrading proteases in tumour invasion and metastasis
1.2.13.1 Matrix Metalloproteinases (MMP)
3 Some degradative enzymes and inhibitors synthesized by malignant and normal cells.
2.Main members of the MMP family
Structure and Mechanism of Activation of MMPs
Role of proteinase Inhibitors in Invasion
1.2.13.2 Serine Proteinases
1.2.13.3 Cysteine proteinases
1.2.13.4 Aspartyl proteinases
1.2.14 Angiogenesis
4.Different mechanisms involved in the switch to the angiogenic phenotype.
3.Angiogenic and anti-angiogenic peptides
1.2.15 Metastasis-Genetic control
1.2.16 Role of polyphenolic compounds in cancer prevention
Flavonoids
5 Structure of polyphenolic compounds.
Catechin and Epicatechin
Quercetin, Rutin and Morin
Naringin and Naringenin
Genistein and Daidzein
Phenolic acids
Curcumin
Ellagic Acid
Rasayanas
1.2.17 Relevance of present study
2. MATERIALS AND METHODS
2.1 MATERIALS
2.1.1 Test compounds
2.1.2 Chemicals
2.1.3 Reagents
2.1.4 Instruments and devices
2.15 Animals
2.1.6 Tumour cell lines
2.2 METHODOLOGY
2.2.1 Sterilization of glasswares
2.2.2 Preparation of culture media
2.2.3 Maintenance of cell lines in tissue culture
2.2.4 Maintenance of experimental animals
2.2.5 Maintenance of tumours in animals
2.2.6 Preparation of B 16F-10 melanoma cells for in vivo studies
2.2.7 Preparation of B 16F-10 melanoma cells for in, vitro studies
2.2.8 Determination of cell viability
2.2.9 In vitro cytotoxicity studies
2.2.10 Determination of the effect of compounds on the solidtumour development
2.2.11 Determination of survival rate of tumour bearing animals
2.2.12 Study on the in viuo invasion of B16F-10 melanoma cells inC57BW6 mice.
2.2.13 Determination of the survival rate of metastatic tumourbearing animals.
6. Lungs of metastatic tumour bearing C57BL/6 mice.
2.2.14 Collagen matrix invasion assay
2.2.15 Tumour cell adhesion experiment
2.2.16 Tumour cell motility assay
2.2.17 Gelatin zymography
Principle
Requirements
Procedure
2.2.18 Estimation of protein
Principle
Reagents
Procedure
2.2.19 Estimation of tissue hydroxyproline content
2.2.20 Estimation of serum sialic acid
2.2.21 Histopathological analysis
2.2.22 Two Stage Carcinogenesis
2.2.23 Methylcholanthrene induced chemical carcinogenesis
2.2.24 Preparation of spleen cells
2.2.25 Preparation of peritoneal macrophages
2.2.26 Collection and preparation of SRBC
2.2.27 Trypsinization of SRBC
2.2.28 Determination of circulating antibody titre
2.2.29 Determination of antibody forming cells
2.2.30 Determination of macrophage mediated cytotoxicity
2.2. 31 Statistical analysis
3. CYTOTOXIC AND TUMOUR REDUCING ACTIVITY OF POLYPHENOLIC COMPOUNDS
3.1 INTRODUCTION
3.2 MATERIALS AND METHODS
3.2.1 Test compounds
3.2.2 Tumour cells
3.2.3 In vitro short term cytotoxicity studies
3.2.4 Cytotoxicity of polyphenolic compounds in tissue culture
3.2.5 Effect of polyphenolic compounds on solid tumour development
3.3 RESULTS
3.3.1 In vitro cytot.oxic activity of polyphenolic compounds
4.Short term in vitro cytotoxicity of polyphenolic compounds towards B16F-10 melanoma cells.
5.Short term in vitro cytotoxicity of polyphenoliccompounds towards DLA cells
3.3.2 Cytotoxicity of polyphenolic compounds in tissue culture
6.Short term in vitro cytotoxicity of polyphenolic compounds towards EAT cells
7.Long term in vitro cytotoxicity of polyphenolic compounds towards B16F-10 melanoma cells.
8.Long term in vitro cytotoxicity of polyphenolic compounds towards L929 cells.
7. Effect of polyphenolic compounds on B16F-10 melanoma induced solid tumour development.
3.3.3 Effect of polyphenolic compounds on solid tumour development
3.4 DISCUSSION
9.Rate of survival of solid tumour (B16F-10) hearing animals treated with polyphenolic compounds.
4. EFFECT OF POLYPHENOLIC COMPOUNDS ON LUNG METASTASIS OF B 16F- l0 MELANOMA CELLS IN MICE
4.1 INTRODUCTION
4.2 MATERIALS AND METHODS
4.2.1 Animals
4.2.2 Test compounds
4.2.3 Effect of polyphenolic compounds on the inhibition of lung nodule formation
4.2.4 Statistical analysis
4.3 RESULTS
4.3.1 Effect of polyphenolic compounds on the inhibition of lung metastasis
10.Effect of polyphenolic compounds on the lung tumour nodule inhibition and survivalof metastatic tumour bearing animals.
11.Effect of isoflavones on the lung tumour nodule inhibition and survival of metastatic tumour bearing animals.
4.3.2 Effect of polyphenolic compounds on the survival rate of tumour bearing animals
4.3.3 Effect of polyphenolic compounds on lung collagen hydroxyproline content
12.Effect of polyphenolic compounds on the lung hydroxyproline and serum sialic acid content of metastatic tumour bearing animals.
4.3.4 Effect of polyphenolic compounds on serum sialic acid level
13.Effect of isoflavones on the lung hydroxyproline and serum sialic acid content of metastasic tumour bearing animals.
4.3.5 Histopathological analysis of lungs of metastatic tumour bearing animals
4.4 DISCUSSION
8 Histopathological analysis of lungs of metastatic tumour bearing animals treated with polyphenolic compounds.
5. EFFECT OF POLYPHENOLIC COMPOUNDS ON THE IN VITRO INVASION OF B 16F-10 MELANOMA CELLS THROUGH COLLAGEN MATRIX
5.1 INTRODUCTION
5.2 MATERIALS AND METHODS
5.2.1 Cell line
5.2.2 Polyphenolic compounds
5.2.3 Effect of polyphenolic compounds on the collagen matrix invasion assay
5.2.4 Effect of polyphenolic compounds on gelatin substrate gel electrophoresis
5.2.5 Effect of polyphenolic compounds on tumour cell adhesion to collagen matrix
5.2.6 Effect of polyphenolic compounds on tumour cell motility
5.3 RESULTS
5.3.1 Effect of polyphenolic compounds on collagen matrix invasion assay
14.Effect of polyphenolic compounds on the inhibition of B 16F- 10 melanoma cells across collagen matrix.
9. Effect of polyphenolic compounds on invasion of B 16F- 10 melanoma cells through collagen matrix
5.3.2 Effect of polyphenolic compounds on gelatin substrate gel electrophoresis
10 Zymographic analysis of B 16F-10 melanoma cell lysate.
5.3.3 Effect of polyphenolic compounds on adhesion of B16F-10 cells to collagen matrix
5.2.4 Effect of polyphenolic compounds on B 16F-10 cell motility
5.4 DISCUSSION
6. STUDIES ON ANTICARCINOGENIC ACTIVITY OF POLYPHENOLIC COMPOUNDS IN MICE
6.1 INTRODUCTION
6.2 MATERIALS AND METHODS
6.2.1 Animal
6.2.2 Compounds
6.2.3 Effect of polyphenolic compounds on methylcholanthrene induced chemical carcinogenesis
6.2.4 Effect of polyphenolic compounds on two stage carcinogenesis
6.3 RESULTS
6.3.1 Effect of polyphenolic compounds on the development of sarcoma induced by methylcholanthrene
15.Effect of polyphenolic compounds on
6.3.2 Effect of polyphenolic compounds on the survival of sarcoma bearing animals
6.3.3 Effect of polyphenolic compounds on two stage carcinogenesis
16. Effect of polyphenolic compounds on survival of.sarcoma bearing animals.
17 Effect of polyphenolic compounds on Two Stage Carcinogenesis
11. Effect of polyphenolic compounds on Two Stage Carcinogenesis.
6.3.4 Effect of polyphenolic compounds on the collagen deposition during two stage carcinogenesis
6.4 DISCUSSION
18. Effect of polyphenolic compounds on the onset of Papilloma during Two Stage Carcinogenesis.
19.Effect of polyphenolic compounds on skin collagen hydroxyproline content during carcinogenesis.
7. IMMUNOLOGICAL MECHANISM OF ACTION OF POLYPHENOLIC COMPOUNDS IN MICE
7.1 INTRODUCTION
7.2 MATERIALS AND METHODS
7.2.1 Test Compounds
7.2.2 Animals
7.2.3 Determination of circulating antibody titre
7.2.4 Determination of antibody producing cells
7.2.5 Effect of polyphenolic compounds on the production of TNF- α by macrophages
(a) In vivo activation of macrophages
(b) In vitro activation of macrophages
7.2.6 Effect of polyphenolic compounds on the serum TNF- α levels in metastatic tumour bearing animals
7.3 RESULTS
7.3.1 Effect of polyphenolic compounds on circulating antibody titre
7.3.2 Effect of polyphenolic compounds on antibody producing cells
7.3.3 Effect of polyphenolic compounds on macrophage activation
7.3.4 Effect of polyphenolic compounds on the serum TNF- α production in metastatic tumour bearing animals
20.Effect of polyphenolic compounds on the circulating antibody titre in mice.
21.Effect of polyphenolic compounds on antibody producing cells.
7.4 DISCUSSION
12 Effect of polyphenolic compounds on the serum TNF-α level in metastatic tumour bearing animals.
8. STUDIES ON THE ANTIMETASTATIC ACTIVITIES OF ANTIINFLAMMATORY AGENTS IN MICE
8.1 INTRODUCTION
8.2 MATERIALS AND METHODS
8.2.1 Animals
8.2.2 Cell line
8.2.3 Test compounds
8.2.4 Effect of antiinflammatory agents in the inhibition of lung tumour nodule formation
8.2.5 Statistical analysis
8.3 RESULTS
8.3.1 Effect of antiinflammatory agents on the inhibition of lung metastasis
22.Effect of antiinflammatory agents on BI6F-10 melanoma induced lung metastasis.
8.3.2 Effect of anti inflammatory agents on the survival rate of metastatic tumour bearing animals
8.3.3 Effect of antiinflammatory agents on the lung collagen hydroxy proline content
8.3.4 Effect of antiinflammatory agents on serum sialic acid level
23.Effect of antiinflammatory agents on the rate of survival of metastasic tumour bearing animals.
24.Effect of anti inflammatory agents on the serum sialic acid and lung hydroxyproline content of metastatic tumour bearing animals.
8.3.5 Histopathological analysis
8.4 DISCUSSION
9. ANTICARCINOGENIC AND ANTIMETASTATIC ACTIVITY OF RASAYANAS
9.1 INTRODUCTION
9.2 MATERIALS AND METHODS
9.2.1 Animals
9.2.2 Cell line
9.2.3 Materials
9.2.4 Determination of the effect of Rasayanas in the inhibition of lung tumour nodule formation and survival
9.2.5 Effect of Brahma Rasayana on the development of methylcholanthrene induced chemical carcinogenesis
9.3 RESULTS
9.3.1 Effect of Rasayanas on the inhibition of lung tumour nodule formation
9.3.2 Effect of Rasayanas on the survival of metastatic tumour bearing animals
25.Effect of various Rasayanas on the inhibition of lung tumour nodules.
26.Effect of various Rasayanas on the survival of metastatic tumour bearing animals.
9.3.3 Effect of Rasayanas on the lung collagen hydroxyproline content
9.3.4 Effect of Rasayanas on the serum sialic acid level
27.Effect of various Rasayanas on the serum sialic acid and lung hydroxyproline content of metastic tumour bearing animals.
9.3.5 Histopathological analysis
9.3.6 Effect of Brahma Rasayana administration on sarcoma development
9.3.7 Effect of Brahma Rasayana treatment on the survival of animal injected with methyl cholanthrene
13. Histopathological analysis of lungs of metastatic tumour bearing animals treated with Rasayanas.
28.Effect of Brahma Rasayana on the development of sarcomα
29.Effect of Brahma Rasayana on the survival of animals induced with methylcholanthrene.
9.4 DISCUSSION
10. SUMMARY AND CONCLUSION
BIBLIOGRAPHY
LIST OF PAPERS