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  • TITLE
  • CERTIFICATE 1
  • CERTIFICATE-2
  • CERTIFICATE 3
  • DECLARATION
  • ACKNOWLEDGEMENT
  • CONTENTS
  • ABBREVIATIONS
  • 1. INTRODUCTION AND REVIEW OF LITERATURE
  • 1.1 Biosynthesis of Rubber from Sucrose
  • 1.2. Factors influencing metabolism of laticifers
  • 13. Factors related to mechanism of latex flow and production
  • 1.4. Clonal variations in carbohydrate metabolism and pH balance
  • 1.5. Factors involved in the latex regeneration mechanism in Hevea latex
  • 1.6. Latex regeneration metabolism and isoprene synthesis
  • 1.7. Phospholipids, glycolipids and latex production
  • 1.8. Membrane enzymes involved in proton transport
  • 1.9. Enzymes involved in latex production through coagulation
  • 1.10. Carbohydrate metabolism and latex flow
  • 2. MATERIALS AND METHODS
  • 2.1. Location
  • 2.2.1 Plant material for experiment
  • 2.3 Latex Collection and seperation of Different Fractions for Biochemical Analysis
  • 2.4 Dry Rubber Yield
  • 2.5 DRC
  • 2.6 Latex PH
  • 2.7 C-and B-Serum PH
  • 2.8 Amount of C-Serum, Rubber Cream and Bottom Fraction
  • 2.9 Busting Index (BI) of Lutoids
  • 2.10 Estimation of Thiols, Inorganic Phosphorous and Sucrose in Latex
  • 2.11 Lipid Extraction from Latex, Rubber Cream and Bottom Fraction
  • 2.12 Estimation of Phospholipids
  • 2.13 Estimation of Glycolipid
  • 2.14 Estimation of Sialic acid B Serum
  • 2.15 Estimation of Protien Bound Hexose and Fucose in B-Serum
  • 2.16 Estimation of Sucrose Synthase Activity in C-Serum
  • 2.17 Estimation of Invertase Activity in C-Serum
  • 2.18 ATP ase Activity of Lutoids
  • 2.19 Pyrophosphatase Activity of C-Serum
  • 2.20 Pyrophosphatase Activity of Lutoids
  • 2.21 N-Acetyl Glucosaminidase in B-Serum
  • 2.22 Chitinase Activity in B-Serum
  • 2.23 Hevein Content of B-Serum
  • 2.24 Estimation of Pyrophosphate
  • 2.25 Estimation of total protein in C-and B-serum
  • 2.26. Extraction of bark samples for biochemical analysis
  • 3. RESULTS
  • 3.1 Variations in Physiological and Biochemical Parameters Associated with Latex Production
  • 3.2 Seasonal variations in carbohydrates and related enzymes associated with latex production in clone RRII 105 under 1/2s d/2 6d/7 system of tapping
  • 3.3. Clonal variations in parameters associated with latex production
  • 3.4. Physiological and biochemical parameters related to carbohydrate metabolism and ionic balance under 1/2 S d/2 6d/7and 1/2 S d/4 6d/7 tapping system in clone RRII 105
  • 3.5. Effect of stimulation on carbohydrate metabolism and ionic balance in clone RRII 105 under 1/2S d/4 6d/7 system of Tapping
  • 3.6. Biochemical parameters related to carbohydrate metabolism in trees affected by TPD
  • 4. DISCUSSION
  • 4.1. Seasonal variations in yield and other physiological parameters related to yield
  • 4.2. Seasonal variation carbohydrates and related enzymes associated with latex production
  • 4.3.Clonal variations in parameters associated with latex production
  • 4.4. Parameters related to carbohydrate metabolism and ionic balance under d/2and d/4 system.
  • 4.5. Effect of stimulation on different biochemical parameters (related tocarbohydrate metabolism and pH Regulation) in clone RRII 105 under d/4 system of tapping.
  • 4.6. Changes in biochemical parameters related to carbohydrate metabolism in TPD affected trees
  • CONCLUSION
  • SUMMARY
  • REFERENCES