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Page: 193
 
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  • TITLE
  • DEDICATION
  • CERTIFICATE
  • DECLARATION
  • ACKNOWLEDGEMENT
  • CONTENTS
  • 1. Introduction
  • 2. Review of Literature
  • 3. Materials and methods
  • 3.1 Spirometry
  • Parameters studied
  • Lung Flow rates
  • 3.2 Blood Parameters
  • 3.2.a Collection ond processing of Blood sample
  • 3.2.b Processing of blood samples
  • 3.2.c Preparation of packed red cell
  • 3.2 d. Preparation of 1: 20 haemolysate
  • 3.3. Storage Stability of Reagents
  • Reagents stable for months at 4°C
  • Reagents to be prepared daily
  • 3.4 Instrumentation
  • 3.5 Calculation of Results
  • A. Method of expressing quantities of red cells
  • B. Calculation of enzyme activities
  • 3.6 Estimation of Haemoglobin
  • 3.7. Estimation of Superoxide Dismutase (SOD)
  • 3.8 Estimation of Reduced glutathione Leuel (GSH)
  • 3.9 Estimation of Catalase Activity
  • 3.10 Estimation of Malone dialdehyde
  • 3. 11 Statistical Analysis
  • 4. Result and discussion
  • 4.1 Respiratory functions (large group)
  • Lung functions of subjects exposed to automobile exhausts, compared to controls
  • DISCUSSION
  • Lung Functions of Subjects Exposed to Automobile Exhausts Based on Duration of Exposure
  • DISCUSSION
  • 4.2 Respiratory functions (small group)
  • Respiratory Functions of Subjects (small group) Exposed to Automobile exhausts in comparison to Controls
  • Respiratory Functions of Subjects (small group) Exposed to Automobile exhausts based on duration of exposure
  • DISCUSSION
  • 4.3 Haematological Parameters based on Age and Exposure
  • DISCUSSION
  • 4.4 Biochemical Parameters based on Age and Exposure
  • DISCUSSION
  • 4.5 General discussion
  • 5. Summary and conclusion
  • BIBILIOGRAPHY
  • APPENDICES
  • APPENDIX I VC of controls & automobiles exhaust exposed smokers& nonsmokers
  • FVC of Control & automobile exhaust exposed Ismokers &nonsmokers
  • MVV of controls & exhaust exposed smokers & ionsm smokers
  • FEV, of controls & automobile exhaust exposedsmokers & nonsmokers
  • PEF Of controls & and automobile exhaustI exposed smokers & non smokers
  • Total WBC count of controls & automobile exhaust exposed smokers & nonsmokers
  • Lymphocyte count of controls & automobile exhaust exposed smokers & nonsmokers
  • GSH of controls & automobile exhaust exposed smokers & nonsmokers (small group)
  • APPENDIX II VITALOGRAPH COMPACT - 11 SPIROMETER
  • APPENDIX III Proforma lmpact of Automoblie Pollution on Pulmonary Functions and Anti Oxidants on Man