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Full Screen- Title
- Declaration
- Certificate (Guide)
- Certificate (Director)
- Acknowledgement
- Abstract
- Preface
- Contents
- List of Tables
- List of Figures
- Abbreviations
- 1. Introduction
- 2. Review of literature
- 2.1 Human Serum Albumin
- 2.2 Recombinant Human Serum Albumin (rHSA)
- 2.3 Microalgae
- 2.4. Chlamydomonas reinhardtii
- 3. Materials and Methods
- 3.1. Culture media and growth conditions for Chlamydomonas reinhardtii.
- 3.3. Vector construction for Chlamydomonas reinhardtii
- 3.4. Transformation of Agrobacterium tumefaciens with the binary vector pSBS.HSA
- 3.5. Genetic transformation of Nicotiana tabaccum
- 3. 6. Agrobacterium mediated genetic transformation of Chlamydomonas reinhardtii with the gene encoding Human Serum Albumin
- 4. Results
- 4.1. Culture conditions of Chlamydomonas reinhardtii
- 4.2. Vector construction of Chlamydomonas reinhardtii
- 4.3. Transformation of Agrobacterium tumefaciens with binary vector Constructs
- 4.4. Transformation of Nicotiana tabaccum
- 4.5. Molecular confirmation of transformation in Nicotiana tabaccum by PCR
- 4.6. Expression analysis of HSA gene by RT-PCR
- 4.7. Transformation of Chlamydomonas reinhardtii
- 5. Discussion
- 6. Summary and Conclusion
- Bibliography